没药中挥发性成分的酶提取及GC/MS分析

Analysis of Cellulase Extraction from the Volatile Components of Myrrha by GC/MS

采用纤维素酶提取法(CE)和水蒸气蒸馏提取法(DSE)对没药挥发油成分进行比较研究。利用气相色谱-质谱联用技术对其化学成分进行分析,从中分别鉴定出34种和38种化学成分,用峰面积归一法通过数据处理系统得出各化学成分在挥发油中的百分含量,分别占挥发油总成分的70.05%和67.95%,共同组分中含量最多的是乙酸辛酯和辛醇。

The cellulase was used for extracting the volatile components of Myrrha. The cellulase extraction （CE） method was made a comparison with distillation extraction（DSE） method. The chemical components were separated and identified by GC/MS. The experimental results confirm that 34 and 38 chemical components are identified from Myrrha respectively. The components were analyzed quantitatively by normalization method. The amount of the identified components are account for more than 70.05% and 67.95 % of all the volatile oils respectively. The main co-compounds of them are acetic acid octyl ester and 1-octanol.