电喷雾质谱剖析烷基多苷的组成与结构

Analysis of Alkyl Polyglucosides by Electrospray Ionization Mass Spectrometry

采用电喷雾质谱法剖析非离子型表面活性剂——烷基多苷(APGs)的组成与结构。APGs按照不同的碳链长度和糖基聚合度被离子化:在负离子模式下,各组分主要以脱质子离子[M—H]-的形式被观察到;在正离子模式下,APGs中的各组分能够以质子化分子离子[M+H]+或阳离子加合物[M+NH4]+[、M+Na]+和[M+K]+的形式被检测到。在正、负模式下,均能观察到双分子加合离子峰和碎片离子峰,其归属分别被讨论。ESI-MS法可快速定性分析APGs的碳链长度和单苷、二苷、多苷的分布情况,以及推测APGs的合成途径(两步法或一步法),可作为一种APGs产品质量检测和控制的快速分析方法。

The ideiatification of different components in raw alkyl polyglucosides （APGs） mixture were achieved by ESI-MS method. The APGs were ionized according to their different alkyl chain lengths and number of glucoside units. Individual components could be detected as cationized ions [M＋H]＋, [M＋NH4]＋, [M＋Na]＋ or [M＋K]＋ in positive ion mode and as deprotonated ions in negative ion mode. In both ion modes the solute-solute clusters and fragments ions could be observed and their assignment was discussed. The distribution of alkyl chain length and polymerization degree of glucoside units could be qualitatively analyzed and the preparation approach （direct synthesis or transacetalization process） of APGs could be known. The highly sensitive ESI-MS analysis technique suits to rapid detection and quality control of APGs products.