高压氧对兔视网膜静脉阻塞后玻璃体氧分压与视网膜血流量的影响

Effects of hyperbaric oxygenation on vitreous oxygen tension and retinal blood flow of experimental retinal vein occluion in rabbits

目的研究实验性视网膜静脉阻塞眼在高压氧(HBO)状态下及HBO暴露后不同时间内玻璃体氧分压与视网膜血流量的变化,以指导HBO治疗的临床应用。方法应用氩激光制作兔眼视网膜静脉阻塞模型,采用血气分析法和放射微球标记法分别测模型眼在高压氧暴露1 h,出氧舱后1、2、4及20 h时的玻璃体氧分压和视网膜血流量。结果HBO状态下,模型眼玻璃体氧分压[(175.233±15.016)mm Hg]较对照组[(94.500±14.438)mm Hg)]明显升高(P<0.01);出氧舱1 h氧分压即有下降趋势,但仍高于对照组(P<0.01);出氧舱2 h的氧分压继续下降,与对照组相比仍有统计学意义(P<0.01);出氧舱4 h的氧分压与对照组相比已无统计学意义(P>0.05)。HBO状态下,模型眼视网膜血流量[(3.385±1.513)ml·min^(-1)·g^(-1)]较对照组[(0.256±0.083)ml·min^(-1)·g^(-1)]明显升高(P<0.01);4 h后继续升高,且已高于对照组(P<0.05);出氧舱后20 h,血流量升至对照组的3倍(P<0.01)。结论HBO状态下模型眼玻璃体氧分压升高,HBO暴露后视网膜血流量逐步增加,有助于实验性视网膜静脉阻塞眼局部血流量的恢复。

Objective To study the changes of xitreous oxygen tension （PO2 value） and retinal blood flow of experimental retinal vein occlusion （RVO） eyes in and after hyperbaric oxygenation treatment（HBOT） for clinical applications. Methods Experimental RVO was done by Argon laser. The samples were taken from the HBOT eyes at different time,PO2 value was determined by blood gas analysis, blood flow was measured with radiolabelled microspheres method. Results In HBOT, vitreous PO2 value [ （ 175. 233 ± 15. 016 ） mm Hg ] markedly increased than that in control group[ （94. 500 ± 14. 438） mm Hg,P 〈0.01 ] ,then kept on decreasing after 1 and 2 hours out of oxygen cabin showing statistical significance （ P 〈 0.01 ）. after 4 hours out of oxygen cabin, there was no statistical significance （ P 〉 0.05 ）. At the same time, retinal blood in rentinal blood flow [ （3. 385 ± 1.1513 ） mm Hg] obviously increased than that in control group [ （ 0. 256 ± 0. 083 ） mm Hg, P 〈 0. 01 ], then kept on rising, obviously increased after 4 hours of HBOT （ P 〈 0.05 ） and three times higher than that of control group （ P 〈 0.01 ）. Conclusions Ocular PO2 value markedly increase in HBOT, after HBOT blood flow keep on increasing gradually and more than that of control group, which is help for recovery of retinal blood flow in experimental retinal vein occluion.