葡萄糖对MG63细胞株TRAIL表达的影响

Effects of glucose on expression of tumor necrosis factor-related apoptosis-inducing ligand in MG63 cells

目的:观察在不同浓度葡萄糖环境下人成骨肉瘤MG63细胞株肿瘤坏死因子相关凋亡诱导配体(TRAIL)及其护骨素(OPG)、护骨素配体(OPGL)的表达,探讨糖尿病骨质疏松症的发病机制。方法:用不同浓度葡萄糖(5.5、16.7、33.3mmol/L)分别刺激培养的MG63细胞24h,采用RT-PCR法检测TRAIL、OPG、OPGLmRNA的表达,以免疫组织化学法检测MG63细胞中TRAIL的表达及分布。结果:不同浓度葡萄糖环境的MG63细胞中TRAIL、OPGLmRNA的表达,均按照对照组、5.5mmol/L组、16.7mmol/L组、33.3mmol/L组的顺序递增(P<0.05),OPGmRNA的表达按照此顺序递减(P<0.05)。33.3mmol/L组TRAILmRNA的水平明显高于对照组[(1.004±0.070)vs(0.740±0.023),P<0.05],TRAIL免疫反应阳性物质密度也明显高于对照组。结论:高糖环境可能导致成骨细胞中TRAIL和OPGL表达增多,OPG表达减少。这可能是糖尿病骨质疏松症的重要发病机制之一。

Objective： To observe the effects of different concentrations of glucose on the expression of tumor necrosis factor-related apoptosis-inducing ligand （TRAIL）, osteoprotegerin （OPG）, and the ligand of osteoprotegerin （OPGL） in osteosarcoma MG63 cells, so as to assess the role of glucose in pathogenesis of diabetic osteoporosis. Methods： MG63 cells were incubated with glucose at the concentrations of 5.5,16.7,33.3 mmol/L for 24 h. The expression of TRAIL, OPG and OPGL mRNA were examined by RT-PCR. Expression and distribution of TRAIL in MG63 cells were investigated by immunohistochemical method. Results： The mRNA expression of TRAIL and OPGL in MG63 cells increased in the order of control group, 5.5,16.7,33.3 mmol/L glucose groups（ P〈 0.05）, and the expression of OPG mRNA decreased in the same order（P〈0.05）. TRAIL mRNA level in 33. 3 mmol/L glucose group was significantly higher than that in the control group [（1. 004±0. 070） vs （0. 740±0. 023）,P〈0.05] and the expression of TRAIL was stronger than that in the control group. Conclusion： High concentration of glucose can lead to increased expression of TRAIL and OPGL in osteoblasts and decreased expression of OPG, which may be one of the key pathogenetic factors of diabetic osteoporosis.