姜黄素减轻沙土鼠海马CA1区脑缺血再灌注损伤与热休克蛋白表达变化的关系

The relationship between the effects of curcumin on cerebral ischemic-reperfusion injury and heat shock protein expressions in hippocampal CA1 area and its significance in gerbils

目的:探讨姜黄素对缺血再灌注沙土鼠脑海马CA1区热休克蛋白(Hsp)基因表达的影响及意义。方法:采用沙土鼠双侧颈总动脉阻断缺血再灌注损伤模型。随机分为假手术组(SH)、脑缺血再灌注组(IR)、姜黄素组(CU)、溶剂对照组(SC);每组据再灌注时间点不同又分6h、1d、3d、5d及7d5个亚组,每组6只动物。在预定时间点行开阔法行为学检查,以TUNEL法行海马CA1区凋亡细胞检测,免疫组化ABC法测定Hsp70、Hsp27基因表达产物Hsp70及Hsp27蛋白在海马CA1区的动态变化。结果:姜黄素可显著减少沙土鼠探索活动及海马CA1区凋亡锥体细胞数量(与IR组相比,P<0.01),进一步诱导Hsp70蛋白表达并抑制Hsp27蛋白的表达(与IR组相比,P<0.01)。结论:姜黄素对脑缺血再灌注损伤有保护作用,调控热休克基因hsp70和hsp27的表达可能是其作用机制之一。

Objective：To explore the relationship between the effects of curcumin on cerebral ischemic-reperfusion injury and heat shock protein genes expressions of hsp70 and hsp27 in hippocampal CA1 area. Methods：Gerbils were randomly divided into sham group （SH）, ischemia-reperfusion group （IR）, curcumin group （CU） and solvent control group （SC）. Forebrain ischemia was induced by occlusion of bilateral common carotid arteries. Observations were carried out in each group at 6h, 1 d, 3 d, 5 d and 7 d after ischemia. Open field test was used to examine the behavioral changes, the apoptosis neurons in hill CA1 region was counted, the expression of Hsp70 and Hsp27 in hippocampal CA1 was detected with SABC immunocytochemical technique. Results：The behavioral mark and the number of apoptosis neurons in hippocampal CA1 region were much less in CU group than that in IR group （P〈0.01）, and the expression of Hsp70 was increased and Hsp27 was decreased in CA1 area in CU group than that in IR group （P〈0.01）. Conclusion：Curcumin can significantly protect neurons against cerebral ischemia, increasing the expression Hsp70 and decreasing the expression of Hsp27 may be the protective mechanisms.