鼻咽癌患者外周血TGF-α和TGF-β1水平的临床意义

Clinical Significance of Serum TGF-α and TGF-β1 Detection in Nasopharyngeal Carcinoma.

目的 本研究对不同分化以及病期等不同类型的鼻咽癌患者外周血TGF-α和TGF-β1的含量及其放疗前后变化进行分析，并探讨其临床意义。方法 采用酶联免疫吸附实验方法对52例明确诊断的鼻咽癌患者放疗前后以及10名健康献血员外周血TGF-β1水平进行测定，并分别对其水平的改变与鼻咽癌的辅助诊断、分化程度、临床分期、近期疗效、年龄、性别进行对比分析。结果 鼻咽癌患者血清TGF-α和TGF-β1水平均明显高于正常人。放疗后血清TGF-α和TGF-β1水平均较放疗前明显降低，且肿瘤分化程度低的患者明显高于分化程度高者，随着临床分期级别的升高，水平也逐渐升高。患者外周血TGF-α和TGF-β1水平与年龄和性别无关。结论 鼻咽癌患者外周血清TGF-α和TGF-β1水平的测定可以为临床诊断提供依据，并能监测鼻咽癌放疗疗效和预后。

Objective This research will analyse the content of transforming growth factor-alpha（TGF-α） and transforming growth factor-betal （TGF-β1）in nasopharyngeal serum in patients with nasopharyngeal carcinoma （NPC） and its clinical significance.Methods Blood of vein was collected from 10 controls and 52 newly diagnosed nasopharyngeal carcinoma patients.The content of TGF-α in nasopharyngeal serum was determined by radioimmunoassay （RIA） in 10 controls and 52 newly diagnosed nasopharyngeal carcinoma patients. The content of TGF-β1 in nasopharyngeal serum was determined by enzyme-lined immuno-sorbent assay（ELISA） in 10 controls and 52 newly diagnosed nasopharyngeal carcinoma patients.Serum samples for TGF-α and TGF-β1 were obtained before and after RT. Measure and analyse the changes of serum TGF-α and TGF-β1 levels and the changes were compared with the clinical diagnose, differentiation degree, clinical substage and immediate response to RT.Results The levels of TGF-α and TGF-β1 in nasopharyngeal serum in patients with nasopharyngeal carcinoma were higher that in control group,and the level was related to the carcinoma differentiation degree and the clinical substage,There is significant correlation between the serum TGF-α level and the clinical substage and immediate response to RT.But the correlation with immediate response to RT was not found in the changes of the level of serum TGF-β1 in patients after RT.Neither TGF-α nor TGF-β1 serum level has related with the sex and age of the patients.These results suggest that the serum TGF level measured by RIA and ELISA may be two useful markers to help to clinical disagnose, monitoring the response and prognosis after RT and a clue to adjuvant treatment in patients with nasopharyngeal carcinoma.Conclusion The level of TGF-α and TGF-β1 in nasopharyngeal serum in patients can offer evident for diagnosis ,and can supervise the curative effect and prognosis of radiationtherapy.