摘要
羟乙基淀粉是一种优良的血浆代用品,作者介绍了用凝胶渗透色谱(GPC)-示差检测(R I)-多角度激光光散射(MALLS)联用技术测试羟乙基淀粉的分子量和分子量分布的实验技术,给出了测试折光指数增量dn/d c值、分子量和分子量分布实验步骤和方法,并对其分析测试过程中的关键实验技术及实验结果进行了详细的讨论。该方法以CH3COOH/CH3COONa溶液作为流动相,实验获得了满意的结果,可作为羟乙基淀粉分子量和分子量分布测试的常规分析方法。
The author introduces a method and experimental procedures that determine dn/dc , molecular weight and its distribution of the hyroxyethyl starch (HES) by means of Gel Permeation Chromatography- Refractive index- Multi- angle laser light scattering detection (GPC-RI-MALLS)techniques. The key experimental technique and results are discussed. The method takes CH3 COOH/CHaCOONa as mobile phase and solvent . The experiment gets satisfactory results. It can be used as a routine method to determine the hyroxyethyl starch (HES) molecular weight and its distribution.
出处
《重庆大学学报(自然科学版)》
EI
CAS
CSCD
北大核心
2007年第7期121-123,129,共4页
Journal of Chongqing University
基金
国家自然科学基金(3027039550305037)
重庆大学创新基金资助项目
关键词
激光光散射法
GPC
羟乙基淀粉
代血浆产品
分子量
分子量分布
测定
laser light scattering method
the hyroxyethyl starch (HES)
plasma substitutes
GPC
molecular weight
molecular mass distribution
determination