参麦注射液对急性心肌梗塞大鼠血浆及心肌组织中ANP含量的影响

Effects of Shenmai Injection on ANP in the Blood Plasma and Cardiac Muscle after Acute Myocardial Infarction in Rats

目的探讨参麦注射液对急性心肌梗塞(AMI)大鼠血浆及心肌组织中心房钠尿肽(ANP)含量的影响。方法通过结扎冠状动脉前降支建立AMI模型大鼠,AMI后24 h存活的48只大鼠随机分为4组:AMI后1周模型组、AMI后参麦注射液治疗1周组、AMI后2周模型组、AMI后参麦注射液治疗2周组。另设1周、2周假手术组各8只。治疗组腹腔注射参麦注射液1次/d,AMI模型组和假手术组大鼠腹腔注射等量的生理盐水。测量各组血浆及心肌组织中ANP含量。结果与假手术组比较,AMI后1周时,血浆及心肌组织中ANP含量明显增高(P<0.01);到梗塞后2周时,虽有所降低,但明显高于模型组,有显著性差异(P<0.01)。参麦注射液治疗组血浆及心肌组织中ANP含量降低,与相应模型组相比差异显著(P<0.05)。结论心肌梗塞后,不论血液循环还是局部心肌组织中ANP都显著性增高,参麦注射液能明显降低ANP的作用。

Objective To evaluate the effects of Shenmai injection on ANP in the blood plasma and cardiac muscle after acute myocardial infarction（AMI） in rats. Methods AMI model was established by ligation of the anterior descending coronary artery in male SD rats. Twenty-four hours after the procedure, the 48 surviving rats were grouped randomly as AMI a week group, AMI two weeks group,Shenmai injection treatment a week and two weeks group （n = 12）. A week sham-operated group and two weeks sham - operated group were taken randomly as non - infarction control. A week or two weeks after treatment with the drug and placebo by intraperitoneally（IP） , the ANP in the blood plasma and cardiac muscle was assessed. Results Compared with sham-operated group , ANP in the blood plasma and cardiac muscle increased significantly in AMI group after a week（ P 〈 0.01 ） ;ANP decreased in AMI group after two weeks ,but higher than AMI group（ P 〈 0.01 ）. In comparison with AMI group , ANP decreased significantly in Shenmai injection treatment group after two weeks（ P 〈 0.05 ）. Conclusion ANP in the blood plasma and cardiac muscle after acute myocardial infarction （ AMI ） in rats increase significantly, ANP decrease significantly in Shenmai injection treatment group.