高效液相色谱法测定沉香药材中两种活性成分的含量

HPLC Determination of 2 Active Components in Aquilaria sinensis (Lour.) Gilg

目的建立高效液相色谱法测定沉香药材中6-羟基-2-[2-(4'-甲氧基苯乙基)]色酮(A),6-羟基-2-(2-苯乙基)色酮(B)2种活性成分的含量。方法采用HPLC法,色谱柱为D iamonsilTMRP-18色谱柱(250 mm×4.6 mm,5μm);流动相为乙腈-甲醇-水(27∶27∶46),流速0.8 ml.min-1;柱温35℃;检测波长226 nm。结果6-羟基-2-[2-(4'-甲氧基苯乙基)]色酮(A)在0.070 8~0.354 mg范围内与峰面积呈良好的线性关系,平均回收率为97.75%,RSD为1.14%;6-羟基-2-(2-苯乙基)色酮(B)在0.232 mg^16 mg范围内与峰面积呈良好的线性关系,平均回收率为98.40%,RSD为1.29%。结论该方法简便、准确,可用于沉香药材中6-羟基-2-[2-(4'-甲氧基苯乙基)]色酮(A)和6-羟基-2-(2-苯乙基)色酮(B)的含量测定。

Objective To establish an HPLC method for the determination of 6-hydroxy-2-[ 2 - （ 4＇-methoxyl-phenyl ） ethyl ] chromone and 6-hydroxy-2-（2-phenylethyl） chromone in Aquilaria sinensls （Lout,） Gilg. Methods The determination was performed on a DiamonsilTMRP- 18 column, The mobile phase was acetonitrile- methanol- water solution（ 27： 27： 46） and UV detection wavelength was at 226 nm, The flow rate was 0.8 ml·min^-1 and the column temperature was at 35℃. Results The linear ranges of 6 - hydroxy-2- [ 2- （4 ：methoxyl-phenyl） ethyl ] chromone and 6-hydroxy-2- （2-phenylethyl） chromone were 0. 070 8 - 0.354 mg（r = 0.999 8）, 0. 232 - 1.16 mg（r = 0.999 7） , respectively. The average recovery rates were 97.75% （RSD = 1.14% ）, 98. 40% （ RSD = 1.29% ） respectively. Conclusion This method is simple, accurate and can be used to determine the contents of 6 - hydroxy -2- [ 2 - （ 4 ：methoxyl -phenyl ） ethyl ] chromone （ A ） and 6 -hydroxy -2 - （ 2-phenylethyl ） chromone （ B ） in Aquilaria sinensis （Lour.） Gilg.