摘要
通过生物信息学同源分析,在水稻cDNA数据库中检索到水稻Dof家族中的OsDof-13(AK119803)基因与玉米MNB1a转录因子具有很高的同源性。NCBI数据库注释,该基因编码202个氨基酸。依据Dof转录因子共有的特征,N端51~102AA可能为该基因的Dof结构域。Northern分析结果表明Os-Dof-13受低氮诱导后有明显的表达变化。亚细胞定位分析结果表明OsDof-13蛋白定位于细胞核。构建了OsDof-13的超表达载体,利用农杆菌介导法将其转化粳稻品种合江19,获得12个独立转基因植株,South-ern杂交结果显示OsDof-13基因的插入拷贝数分别为1~4个。
Bioinformatic analysis shows that OsDof-13 (AK119803), a member office Dof family, is highly homologous to the Dof transcfiption factor MNBla in maize (Zea Mays). In NCBI database OsDof-13 protein consists of 202 amino acids. According to the common character of the Dof transcription factors, the region of 51-102 amino acids at N-terminator of OsDof-13 protein is predicted to be a conserved Dof motif. Northern blot analysis verified that OsDof-13 was strongly induced by low nitrogen stress, while OsDof-13 protein was located in nucleus by sub-cellular localization. An OsDof-13 over-expression vector was constructed and transformed into a japonica rice variety Hejiang 19 by A grogacterium-mediated transformation. Southern blot assay indicated that OsDof-13 was successfully integrated into the Hejiang19 genome with 1-4 gene copies.
出处
《分子植物育种》
CAS
CSCD
2007年第4期455-460,共6页
Molecular Plant Breeding
基金
国家转基因植物研究与产业化开发专项"水稻高产高效等性状改良关键基因利用研究"资助。