摘要
以薜荔茎段为外植体,研究了其丛生芽诱导和快繁技术.实验结果表明,B5为最适合的基本培养基,根据正交试验结果,各激素对诱导丛生芽的作用大小为:6-BA>NAA>KT>IAA,诱导丛生芽的最佳培养基为:B5+1 mg/L NAA+0.1 mg/L IAA+1mg/L KT+2 mg/L 6-BA+蔗糖20 g/L+琼脂0.75%;生根培养基以B5+3 mg/L NAA+0.1 mg/L IAA+1 mg/L KT+2 mg/L6-BA+蔗糖20 g/L+琼脂0.75%为宜.
In this study, we investigated the tissue culture and plantlet regeneration of Ficus pumila L. using its stem segment as the explants. The resultshows that the optimal basic medium suitable for Ficus pumila L. is the B5 medium. Based on the result of orthogonal experiment, the effect of various hormones on induction of clump buds is determined as follows： 6-BA 〉 NAA 〉 KT 〉 IAA, and the optimum medium for inducing clump buds is B5＋ 1 mg/L NAA ＋ 0. 1 mg/L IAA ＋ 1 mg/L KT ＋ 2 mg/L 6-BA ＋ cane sugar 20 g/L＋ agar 0. 75%. For rooting, B5＋ 3 mg/L NAA ＋ 0.1 mg/L IAA ＋ 1 mg/L KT ＋ 2 mg/L 6-BA ＋ cane sugar 20 g/L ＋ agar 0. 75% has a better effect.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2007年第3期10-13,共4页
Journal of Central South University of Forestry & Technology
基金
国家"948"项目"薜荔新品种选育及其栽培利用技术的引进"(编号:2003-4-15)的部分内容
关键词
薜荔
组织培养
快速繁殖
茎段
正交试验
Ficus pumila L.
tissue culture
rapid regeneration
stem segment
orthogonal experiment
