棉株植物内生菌诱导棉花抗黄萎病过程中同工酶活性的变化

Isoenzymatic Activities Involved in Induced Resistance of Cotton to Verticillium dahliae by Endophytic Bacteria

用聚丙烯酰胺凝胶电泳法，研究接种植物内生性生防菌后，其诱导棉花抗黄萎病过程中棉花茎叶组织中的过氧化物酶、超氧化物歧化酶（ＳＯＤ）和酯酶活性的变化。①诱导接种植物内生性生防菌７３ａ后，接种点附近棉茎中的过氧化物酶活性高，表现为酶带宽，颜色深，活性明显强于单用针剌的对照，而针剌对照又明显高于空白对照；接种后４天，接种７３ａ等内生性生防菌的酶谱比针剌对照及空白对照多１条Ｒｆ为０．２８的酶带。挑战接种后１０天测定，接种点附近的棉茎间过氧化物酶活性同对照已无明显差异。棉花叶片组织测定表明，诱导接种４天后，接种内生菌７３ａ的叶片过氧化物酶活性强于针剌对照。②诱导接种７３ａ后２天，接种点附近的棉茎中ＳＯＤ酶谱有３条酶带，多于空白对照的２条，且活性强。③诱导接种７３ａ后２４小时，接种点附近棉茎组织酯酶酶谱比空白对照多１条Ｒｆ为０．

The activities of peroxidase, SOD and esterase isoenzymes were studied after inductive inoculation of endophytic bacteria and challenge inoculation of Verticillium dahliae . Cotton stems inoculated with bacterium 73a, showed higher isoenzymatic activites and exposed one more band in zymogram at 4 day after challenge inoculation as compared with two control stems: needle puncture and without treatment. The leaves inoculated with bacterium 73a, began to show slightly higher peroxidase activities than the blank control until 4 days after inductive inoculation. The stems inoculated with 73a demonstrated higher SOD and esterase isoenzymatic activites and exposed one band more than no inoculation stems in zymogram.