秸秆纤维素分解菌的酶活力测定

Determination of Enzyme Activity of Straw Cellulose-Decomposing Microorganisms

目的:测定秸秆纤维素分解菌的酶活力。方法:从土壤中分离出具有分解纤维素能力的菌株,采用刚果红染色法进行粗选,得到7株透明圈较大的菌株。将这7株菌株液体发酵培养6d,再分别用滤纸分解度观察、羧甲基纤维素酶活法(CMC)、滤纸酶活法(FPA)和天然纤维素酶活法测定其酶活力。结果:在7株菌株中,F-1、F-2、F-3、F-5的酶活力测定结果与其溶解圈的测定结果、滤纸分解结果基本相同。且天然纤维素酶活力高的菌株,其CMC酶活、FPA酶活也高,滤纸分解效果也比较明显。结论:CMC法、FPA法和天然纤维素酶活法适于测定秸秆纤维素分解菌的酶活力。

Objective： To determine enzyme activity of straw cellulose-decomposing microorganisms. Methods： Some strains of fungi for decomposing cellulose were chosen from soil. After the first filtration, seven colonies surrounded by some distinct zones of clearing were chosen after the pigmentation of Congo red. After six-day′s fermentation in the liquid culture medium, their enzyme activity determined by carboxymethylcellulose（CMC）, filter paper activity（FPA）, natural cellulase activity and decomposition of filter paper. Results： Enzymes activity of natural cellulase, CMC, and FPA in strain F-1, F-2, F-3, and F-5 by direct detection was similar to those by clear zones and decomposition of filter paper respec- tively. The strains with higher natural cellulase activity had higher CMC activity and FPA activity, and the effects of decomposing filter paper were more visible. Conclusion： The CMC, FPA and natural cellulase activity methods are suit for determining the enzyme activity of straw cellulose-decomposing microorganisms.