应用逆转录聚合酶链反应直接检测草鱼出血病病鱼组织的研究

DIRECT DETECTION OF GRASS CARP HEMORRHAGIC VIRUS IN INFECTED FISH BY REVERSE TRANSCRIPTION(RT)POLYMERASE CHAIN REACTION(PCR)

人工感染GCHV－861后，对处于潜伏期、发病期和恢复期等不同时期的草鱼内脏组织匀浆上清液进行逆转录聚合酶链式反应（RT－PCR）扩增，除恢复期的1条草鱼外，其余样品均得到特异扩增带，而对照组都没有，预示着RT－RCR技术对于草鱼出血病的早期诊断、防治及抗病有种具有重要意义。另外，对于显症出血病草鱼的肝、肾、脾、鳃、肌肉和肠道等组织器官进行检测，结果都为阳性，首次证实了GCHV存在于肝脏中，并对此作了进一步的讨论。

After artificial infected with GCHV-861, the grass carp were collected and detected during developing period, pre- and post-developing period by RT-PCR, respectively. All the infected fish gave positive results except one of five piece of covalenscent fish, and the controls are negtive.These indicated tha RT-PCR is significent for the early diagnosis and the viral disease-resistant breeding of grass carp- Moreover, we also first detected GCHV from the liver of infected hemorrhagic grass carp and discussed it.