血清中葡萄糖6-磷酸异构酶在类风湿关节炎中的诊断意义

The diagnostic value of glucose-6-phosphate isomerase in patients with rheumatoid arthritis

目的探讨血清中葡萄糖6-磷酸异构酶(glucose-6-phosphate isomerase,GPI)抗原升高在类风湿关节炎(rheumatoid arthritis,RA)患者诊断中的意义。方法用酶联免疫吸附试验(ELISA)检测128例RA患者,86例其他风湿病患者和42例健康对照血清中GPI抗原的浓度,RA患者同时还检测了类风湿因子(RF),关节疼痛及肿胀数,血沉(ESR),以及X线分级等。结果128例RA患者血清中GPI浓度为(2.52±2.37)μg/ml,86例其他风湿性疾病组为(0.083±0.056)μg/ml,42例健康对照组为(0.081±0.050)μg/ml,RA患者血清中GPI浓度显著高于其他风湿病组和健康对照组(P<0.05),在RA活动组和RA非活动组,亦有显著差异(P<0.05)。通过分段回归分析发现GPI的浓度和RA病情活动成正相关。GPI抗原对RA检测的敏感性为43.5%,特异性为98.3%。结论GPI在部分RA患者血清中显著升高,有可能成为诊断RA及判断其疾病活动性的一个新指标。

To investigate the significance of glucose-6-phosphate isomerase （GPI） expression in patients with rheumatoid arthritis（RA）. Methods The level of serum GPI in 128 patients with RA, 86 patients with inflammatory diseases and 42 normal control group were assayed by ELISA.The level of RF, attic tenderness and engorse, X-Ray＇s classification, and ESR were also assayed in RA patients. Results ults GPI serum level in patients with RA were （2.52 ± 2.37） μg/ml, and were （0. 083 ± 0.056） μg/ml in other inflammatory diseases, and 0.081 ±0.050） /μg/ml in normal control group.There was significant statistical differences between patients with RA and other inflammatory diseases（ P 〈 0.05）, active RA and inactive RA were also have significant statistical differences（ P 〈 0.05）, and GPI serum level was positively associated with the activity of B.A. Conclusion The GPI that abnormally increased in some RA patients may be a new marker for diagnosing RA and accessing its activity.