ELISA法测定血清S100B蛋白

ELISA for Measuring Serum S100B Protein

目的建立ELISA测定血清S100B蛋白的方法。方法制备S100B单克隆和多克隆抗体,以双抗体夹心法测定血清S100B蛋白。结果方法的平均回收率为93.7%-104%,批内及批间平均变异分别为3.7%和5.9%,方法的线性为0-12.5μg/l,参考值范围为0.14-0.38μg/l。结论该法简便、快速、特异、敏感,适于临床常规应用。

Objective To establish ELISA for measuring serum S100B protein. Methods Preparation of monoelonal antibody and polyelonal antibody for the examination of serum S100B protein by ＂sandwich＂ method. Results The analytical reeovery were 93.7% - 104% at 0.1 - 12.5 μg/L of S100B protein.The within-assay and between-assay were 3.7%and 5.9% respectively.The linearity of method was 0- 12.5 μ/L. The range of reference value were 0.14 - 0.38μ/L. Conclusion The method is simple, rapid,specific and sensitive. It＇s suitable for clinical laboratory routine use.