血清铁自动分析法试剂盒的研制与评价:Nitro-PAPS直接光度法

Investigation and evaluation of the reagent kit of automatic analysis serum iron,using Nitro-PAPS direct spectrophotometric method

目的研制、评价血清铁Nitro-PAPS自动分析法试剂盒。方法在表面活性剂存在下,用2-(5-硝基-2-吡啶偶氮)-5-[N-正丙基-N-(3-磺酸丙基)氨基]苯酚二钠(Nitro-PAPS)作显色剂双试剂两点终点法测定血清铁。结果该方法显色络合物最大吸收波长为591 nm,线性范围达85μmol/L,表观摩尔吸光数为1.11×105L.mol-1.cm-1,回收率为98.5%-104.1%,批内变异系数(CV)和批间变异系数分别为0.021和0.035,与原子吸收分光光度法(AAS)比较相关良好,Y=0.997X-0.223,r=0.9786,P>0.05,224例健康人血清铁含量为(10.83-29.07)μmol/L(±2 s)。结论用本试剂盒双试剂自动化分析法测定血清铁方法简便、灵敏可靠,适合临床应用。

Objective To investigate and evaluate the the reagent kit of automatic analysis the zinc in serum.Methods For determination of serum zinc with Nitro-PAPS in the presence of surfactant. Results The method was liner in the range of 0 - 85μmol/L, the rate of recovery was 98.5 % - 104.1%,, the withinrun and between run CV was 0. 021 and 0. 035. Comparing this method （Y） with AAS （X）,we obtained the regression equation： Y = 0. 997 X-0. 223, r = 0. 9786, P 〉 0.05. The reference interval（± 2s） was 10.83- 29.07μmol. L^-1 （ n = 224） Conclusion This reagent kit of automatic analysis the iron in serum is simple, sensitive and suit-able for clinical application.