雌二醇诱发大鼠抑郁状态的实验研究

The experimental study on the female rats'depression statement caused by estradiol benzoate

目的用外源性雌激素造成实验大鼠高雌激素状态，探讨高雌激素与经行抑郁的关系。方法选择3～4月龄成年未孕SD大鼠，随机分为空白组、利血平组、雌激素组，分别对每只动物进行阴道脱落细胞涂片观察，逐只建立动物动情周期档案，选择每只大鼠的动静期开始造模，每日上午皮下注射苯甲酸雌二醇注射液，连续3天。造成实验动物体内高雌激素状态，观察大鼠行为学及中枢神经递质的变化，并与利血平造成的经典抑郁模型进行比较，评价雌激素造模的成功。结果外源性雌激素可造成实验大鼠持续高水平的阴道角化细胞指数，升高血清E2水平、使中枢5-HT、DA浓度降低，导致动物行为学发生变化：敞箱试验中水平运动、垂直运动、清洁次数明显减少，中央格停留时间明显延长，和利血平组相比，改变的程度虽有差异，但变化的趋势却是一样的。说明在大鼠动情后期给予外源性的雌激素，可以造成实验大鼠出现抑郁状态。结论在大鼠动情期给予外源性雌激素可以导致动情后期大鼠出现抑郁的病理状态。

Objective The objective of this dissertation is to investigate the relationship between menstrual depression and high -estrogenic,by introducing experimental rats into high oestrogenic state using estradiol benzoate. Methods We used young animal model of three to four months old impregnated SD female rats and randomly divided the rats into three different experimental groups. Specifically speaking, they are the control group, the reserpine group, the estradiol group. We performed vaginal smear test for each animal, and set up the files for its estrous cycle, then made the model dur- ing the estrous period. We injected estradiol benzoate into experimental rats on three consecutive mornings to introduce high oestrogenic state, and observed rats＇ behavioral changes as well as changes in their central neuro transmitter. We then compared the results with the classic depression model to evaluate the degree of success of oestrogenic modelling. Results Our experiments demonstratethat estradiol benzoate can increase vaginal kerratinized cell index, increase serum E2 contents , decrease brain stem neur - otransmitter 5 - HT and DA concentrations and change the behavior of the animals ： in open field test of the rats, the vertical motion, level motion and frequency of cleaning are decreased. The time of rats staying in the center are significantly increased. Compared with the reserpine group, the trend of change is the same, though the degree of change varies,These results show that exogenetic estradiol can cause depression in experimental rats when given during the late estrous period. Conclusions In conclusion,exogenetic estradiol can cause depression in experimental rats.