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贻贝凝集素生物活性的研究 被引量:4

Biological Activities of Lectin from Sea Mussel Crenomytilus grayanus
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摘要 从海洋无脊椎动物贻贝中分离出的凝集素(CGL),为N-乙酰半乳糖胺/半乳糖(GalNAc/Gal)特异性的凝集素。经SDS-聚丙烯酰胺凝胶电泳测定,其相对分子量为18 000。其血凝活性不依赖钙离子的存在。在植物凝集素(PHA)存在的条件下,CGL具有体外促进小鼠脾细胞增殖的活性。同时,它还具有抑制酶活性,其抑制50%ACE的浓度(IC50)为6.82 mg/m l和促进体外离体兔回肠蠕动的作用。 A Crenomytilus grayanus lectin (CGL) was purified from sea mussel Crenomytilus grayanus. CGL with molecular weight of 18 000 was found to be GalNAc/Gal specificity by SDS-PAGE. The hemagglutinating activity of CGL was independent of the divalent cation Ca^2+. CGL displayed mitogenic effect on BALB/c splenocytes, inhibited angiotensin converting enzyme (ACE) with ICs0 value of 6.82 mg/mL, and improved the peristalsis of a rabbit ileum.
出处 《水产科学》 CAS 北大核心 2007年第7期400-404,共5页 Fisheries Science
基金 辽宁省自然科学基金资助项目(2050885) 辽宁省教育厅资助项目(05L086) 教育部"留学回国人员科研起动基金"资助项目([2006]331) 大连水产学院科研项目(015690)
关键词 凝集素 β-葡糖苷酶抑制剂 脾细胞 回肠 lectin inhibitor of ACE BALB/c splenocyte ileum
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