壳聚糖-明胶-果胶膜对骨髓基质干细胞的细胞行为研究

Growth characteristics of marrow mesenchymal stem cells on the surface of chitosan-gelatin-pectin films

目的考察壳聚糖-明胶-果胶膜对骨髓基质干细胞(MSC)的作用,为组织工程提供一种新的可望用于组织工程的生物材料。方法将MSC接种到壳聚糖-明胶-果胶(CS-Gel-P)膜和壳聚糖-明胶(CS-Gel)膜上,观察其生长形态,用MTT法测定其细胞活性,并考察其碱性磷酸酶活性表达。结果在CS-Gel-P膜上接种的MSC表现出比在CS-Gel膜和组织培养板(TCPS)上更好黏附性能,同时在不同时间点的碱性磷酸酶表达也均高于CS-Gel膜,在两种不同膜上的MSC的细胞活性差异无统计学意义。结论果胶的加入可以促进MSC的黏附和生长,同时更能促进MSC诱导分化为成骨细胞。

Objective To study the effect of chitosan-gelatin-pectin （CS-Gel-P） films on bone marrow mesenchymal cells and develop a prospective novel biomaterials for tissue engineering. Methods Cell morphology was observed with inverted microscope and scanning electron mieroseope（SEM）, the viability of cell was measured through MTT assay. After 2 and 7 days static culture,the activity alkaline phosphatase（ALPase） of cells was detemined. Results The MSC maintained higher attachment ability on the surface of CS-Gel-P network films than that on the CS-Gel network films. The difference of cell viabilities was not significant on the two films. Conclusion It is demonstrated that adding pectin can promote the MSC attachment,growth on biomaterial films and significantly improve osteogenic differentiation in comparison with that on the surface of CS-Gel network films without pectin.