摘要
应用SDS-PAGE、等电聚焦电泳(IEF)及双向电泳三种电泳方法分析牦牛肝片吸虫成虫四种抗原(头抗原-HA、体抗原-BA、体表抗原SA、分泌排泄抗原-ES)。SDS-PAGE结果表明,BA、HA、SA分子量在12~100kD之间,ES在12.3~26kD之间,蛋白质染色BA、HA、SA、ES分别显示25、24、18、9条带;IEF分析肝片吸虫分别得34、33、28、22条带,主要由酸性蛋白质组成,主要谱带在pI4.20~6.55内;双向电泳分析BA、ES多肽斑点为69。
To analyze the property of four different antigens of extracts respectively from the head(HA), body(BA), surface(SA) and excretory products (ES) of the adult fluke, a parasite in Yak, three different types of electrophoresis were applied. As for the abovementioned fact, we had used four types of extracts for staining proteins respectively, they were 25,24,18 and 9 bands in batches. When applied IEF, the four extracts gave 34,33,28 and 22 bands respectively. Finally, the two dimensional electrophoresis in combination with the other two methods have revealed an even high complexity (30 pesticides blot in ES, 69 blots in BA).
出处
《四川草原》
1997年第2期48-51,64,共5页
Journal of Sichuan Grassland
关键词
牦牛
肝片吸虫
抗原
双向电泳
Yak,Fasciola hepatica, antigens, SDS PAGE,IEF,two dimensional electrophoresis