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柞蚕核型多角体病毒宿主载体表达系统的研究现状及展望 被引量：1

Research Status and Prospect of Antheraea pernyi Nucleopoly-hedrovirus (ApNPV) Host/Vector Expression System
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摘要 对构建柞蚕蛹-柞蚕核型多角体病毒(Antheraea pernyi nucleopolyhedrovirus,ApNPV)宿主载体表达系统及利用其生产有用外源基因产物的基本原理、研究进展和应用前景进行了论述。指出该系统为真核生物表达系统,与大肠杆菌等原核生物和其他昆虫杆状病毒表达系统相比,在表达出的目的基因产物特性、经济效益和产业化生产等方面均具有明显的优越性,应用前景广阔。 This paper discussed how to construct the host vector expression system of Chinese oak silkworm pupa/ApNPV and the basic principle, research advance, application prospect by which used to produce useful exogenous products, It was Eukaryotes expression system, comparing with prokaryotes expression system such as E.coli and other baculovirus expression systems, the ex- pressed target products characteristics and economic profit and industrial production were superior. This expression system has extensive appliance and wide foreground.
作者 王学英 石生林 李群
机构地区 沈阳农业大学生物科学技术学院辽宁省农业生物技术重点实验室
出处 《沈阳农业大学学报》 CAS CSCD 北大核心 2007年第3期259-264,共6页 Journal of Shenyang Agricultural University
基金 辽宁省自然科学基金项目(002042) 辽宁省科学技术厅攻关项目(200120881)
关键词 柞蚕蛹 APNPV 克隆 转移载体 重组 柞蚕培养细胞 基因表达 tussah pupa ApNPV clone transfer vector recombination tussah cultured cell gene expression
分类号 S885.1 [农业科学—特种经济动物饲养]
  • 相关文献

参考文献9

  • 1MAEDA S,KAWAI T,OBINATA M,et al.Production of human alpha-interferon in silkworm us-ing a baculovirus vector[J].Nature,1985,315:592-594.
  • 2SMITH G E,SUMMER M D,FRASER M J.Production of human beta interferon in insect cells infected with a baculovirus expression vector[J].Mol Cell Biol,1983,3:2156-2165.
  • 3陈璟,方宏清,周长林,陈惠鹏.昆虫杆状病毒系统表达外源蛋白的糖基化[J].中国生物工程杂志,2004,24(3):1-6. 被引量：5
  • 4FAN Q,LI S,WANG L,et al.The genome sequence of the multinucleocapsid nucleopolyhe-drovirus of the Chinese oak silkworm Antheraea pernyi[J].Virology,2007,(4):27.
  • 5WANG X Y,Shi J K,SHIGETOSHI,et al.Cloning and property analysis of Antheraea pernyi nu-cleopolyhedrovirus (AnpeNPV)[J].International journal of wild silkmoth and silk,2000,5:51-56.
  • 6KOBAYASHI J,ANDO R,WANG X Y,et al.Nucleotide sequence analysis of the polyhedrin gene of A ntheraea pernyi nucleopolyhedrovirus and construction of a transfer vector plasmid[J].International Journal of Wild Silkmoth and Silk,2001,(6):53-58.
  • 7张春发,刘淑珊,范琦,王林美,李文利,李广泽.柞蚕NPV转移载体组建及外源基因在Sf9细胞、柞蚕卵巢原代细胞和蛹体中的表达[J].蚕业科学,1992,18(3):164-172. 被引量：12
  • 8HUANG Y J,WANG X Y,MIYAJIMA S,et al.Efficiency of Antheraea pernyi nucleopolyhedro-virus-mediated protein production in both an established cell line and diapausing pupae of A.Pernyi[J].International Journal of Wild Silkmoth and Silk,2001,6:59-71.
  • 9MAEGAWA K-I,WANG X Y,KOBAYASHI J,et al.Nucleotide sequence analysis of p10 gene of Antheraea pernyi nucleopolyhedrovirus and construction of two transfer vector plasmid[J].International Journal of Wild Silkmoth and Silk,2004,9:53-60.

二级参考文献21

  • 1[1]Breitbach K,Jarvis D L.Improved glycosylation of a foreign protein by Tn5B1-4 cells engineered to express mammalian glycosyltransferase.Biotechnoi Bioeng,2001,74,230～239
  • 2[2]Ailor E,Takahashi N,Tsukamoto Y,et al.N-glycan patterns of human transferring produced in Trichoplusia ni insect cells:Effect of mammalian galactosyltranferase.Glycobiology,2000,10,837～847
  • 3[3]John B L,Jamey D M.A genetic approach to mammalian glycan function.Annu Rev Biochem,2003,72:643～691
  • 4[4]Noboru T,Eric A,Shawn M L,et al.Determination of nucleotides and sugar nucleotides involved in protein glycosylation by high-pertormance anion-exchangechromatography:Sugar nucleotide contents in cultured insect cells and mammalian cells.Anal Biochem,2001,293,129～137
  • 5[5]Satoko W,Takechiro K,Hitomi T,et al.Sialylation of N-glycans on the recombinant proteins expressed by a baculovirus-inscet cell system under β-N-acetylglucosaminidase ihibition.J Bio Chem,2001,277,5090～5093
  • 6[6]Marchal I,Jarvis D L,Cacan R,et al.Glycoproteins from insect cells:sialylated or not?Biol Chem,2001,382:151～159
  • 7[7]Joshi L,Davis T R,Mattu T S,et al.Influence of baculovirus-host cell interactions on complex N-linked glycosylation of a recombinant human protein.Biotechnol Prog,2000,12:650～656
  • 8[8]Joshi L,Shuler M L,Wood H A,et al.Production of a sialylated N-linked glycoprotein in insect cells. Biotechnol Prog, 2001, 5, 822～827
  • 9[9]Laura A P, Christoph E J, Patrick R H, et al. Novel insect cell capable of complex N-glycosylation and sialylation of recombinant proteins.Biotechnol Prog, 2003,19, 185～192
  • 10[10]Altmann F, Staudacher E, Wilsom I B H, et al.Insect cells as host for the expression of recombinant glycoproteins.Glycocon J,1999, 16, 109～123

共引文献15

同被引文献14

  • 1石生林,潘敏慧,鲁成.柞蚕核型多角体病毒PstI-B、C片段克隆及序列分析(英文)[J].中国生物工程杂志,2007,27(1):81-85. 被引量：1
  • 2Blissard G W, Rohrmann G F. Baculovirus diversity and molecular biology[ J ]. Annu Rev Entomol, 1990,35 : 127 - 155.
  • 3Theilmann, D A, Blissard G W, Bonning B, et al. Baculoviridae[C]//Fauquet C M, Mayo M A, Maniloff J, et al. VIIIth report of the International Committee on Taxonomy of Viruses, Elsevier: New York, N Y, 2005 : 177 - 185.
  • 4Shi S L, Pan M H, Lu C. Characterization of Antheraea pernyi nucleopolyhedrovirus pll gene, a homologue of Autographa californica nucleopolyhedrovirus orf108 [ J]. Virus Genes, 2007,35(1) :97 - 101.
  • 5Chen W, Li Z, Li S, et al. Identification of Spodoptera litura multicapsid nucleopolyhedrovirus ORF97, a novel protein associated with envelope of occlusion-derived virus [ J]. Virus Genes, 2006,32 ( 1 ) : 79 - 84.
  • 6Kolaskar A S,Tongaonkar P C. A semi-empirical method for prediction of antigenic determinants on protein antigens [ J ]. FEBS Lett, 1990,276( 1 - 2) : 172 - 174.
  • 7Emanuelsson O, Brunak S, von Heijne G, et al. Locating proteins in the cell using TargetP, SignalP and related tools [ J ]. Nat Protoc ,2007,2(4) :953 - 971.
  • 8Ahschul S F, Madden T L, Schaffer A A, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J]. Nucleic Acids Res, 1997,25 (17) : 3389 - 3402.
  • 9Larkin M A, Blackshields G, Brown N P, et al. Clustal W and Clustal X version 2.0 [J]. Bioinformatics, 2007, 23 (21) : 2947 - 2948.
  • 10Tamura, K, Dudley J, Nei M, et al. MEGA4 : Molecular Evolutionary Genetics Analysis ( MEGA ) software version 4.0 [J]. Mol Biol Evol,2007,24(8) : 1596 - 1599.

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二级引证文献2

沈阳农业大学学报
2007年 第3期

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