摘要
利用微卫星(SSR)标记,在DNA分子水平上对不同地理系统和化性的家蚕品种之间的遗传差异进行了研究。结果表明:在11对引物中均出现稳定的扩增带,扩增带总数为106条,最多的有16条,最少有4条,平均每个引物9.6条。利用单匹配相似系数和UPGMA聚类法对结果进行分析,发现其SSR不但具有品种特异性,而且具有系统特异性,证明不同化性及地理系统的家蚕在进化上属有显著差异的类群。
The aim of the study is to reveal genetic diversity among silkworm varieties with different votinism and belong to different geography systems, based on Simple Sequence Repeat （SSR）. 11 pairs of primers were employed and fragments were amplified stably by each pair of primers. There were 4 - 16 alleles detected by each pair of primers, 106 in total, and the average was 9.6. UPGMA-cluster analysis showed that SSR not only had variety specificity but also system specificity. And it proved that silkworm varieties of different votinism and geography systems belong to significant different groups in phylogeny.
出处
《沈阳农业大学学报》
CAS
CSCD
北大核心
2007年第3期357-361,共5页
Journal of Shenyang Agricultural University
基金
国家科技基础条件平台工作重点项目(2004DKA30460-4)
江苏省自然科学基金前期预研重大项目(BK2004206)
中国科学院知识创新项目(KSCX2-SW-318)
关键词
家蚕
微卫星标记
化性
遗传差异
分子系统树
物种进化
基因图谱
地理种群
Bornbyx rnori
simple sequence repeat （SSR）
voltinism
genetic diversity
phylogenetic tree
species evolution
genetic map
geographical line
