摘要
根据小鼠超高硫角蛋白(UHS)基因已知DNA序列设计合成了两个特异性引物,以小鼠全血提取的总DNA为模板,PCR扩增出688bp的特异性片段,连接到pMD19T载体中获得该片段克隆p19TU。经过快速提取质粒法筛选、限制性内切酶分析证明该克隆就是UHS基因5'端的调控区序列。序列分析结果也表明该克隆片段与原基因调控序列相比具有很高的一致性。研究结果为今后制备转基因克隆动物、在毛囊细胞中特异性表达绒毛生长调控基因奠定了基础。
Two specific PCR primers were designed and synthesized according to the sequence of murine ultra high sulfur keratin （UHS）gene from GenBank,The size of 688bp of UHS promoter sequence was amplified by PCR from mouse total DNA which was prepared from whole blood sample, and the fragment was integrated into EcoRV site of pMD19-T vector. After the sequence of clone pl9TU was compared with the genomic DNA, the result showed that the sequence kept high percentage of identity with original genome. This study made a foundation to producing transgenic animals and the research of the genes regulating the hair follicles.
出处
《科技通报》
2007年第4期479-482,486,共5页
Bulletin of Science and Technology
基金
国家863计划(2002AA242061)
内蒙古自然科学基金(200607010405)
内蒙古自治区高等学校科学研究项目(NJ06055)资助
关键词
小鼠超高硫角蛋白基因
调控区
PCR
序列分析
murine ultra high sulfur keratin gene
regulatory region
PCR
sequence analysis
