摘要
目的:研究去甲斑蝥素对人高转移卵巢癌HO-8910PM细胞中非类固醇抗炎药物激活基因(non-steroidal anti-in-flammatory drug-activated gene,NAG-1)表达的影响,探讨其抗肿瘤的作用机理。方法:应用肿瘤基因芯片、荧光定量实时PCR和Western blot法检测去甲斑蝥素对NAG-1表达的影响。结果:去甲斑蝥素能明显上调NAG-1表达,3种方法检测去甲斑蝥素上调HO-8910PM细胞中NAG-1表达的倍数分别为6.69、8.33±0.76和16.00倍。结论:去甲斑蝥素抗肿瘤作用与NAG-1表达有关。
Aim：To investigate effect and its mechanism of norcantharidin on the expression of nonsteroidal anti-infla mmatory drug-activated gene（NAG-1） in the highly metastatic ovarian carcinoma HO-8910PM cells. Methods： The expression level of NAG-1 was assessed by using the mieroarray chip. Quantitative real-time fluorescent PCR assay and Western blot were used to verify the effect of norcantharidin on NAG-1 expression. Results： Microarray chip, fluores- cent quantitative real-time PCR and Western blot results showed that norantharidin significantly up-regulated the expression of NAG- 1, the relative mRNA and protein level of NAG- 1 were 6.69, 8.33± 0.76, 14.00 fold of control group, respectively. Conclusion： Anti-tumor activity and its mechanism of norcantharidin in HO-8910PM cells are associated with the expression of NAG-1.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2007年第4期356-359,共4页
Journal of China Pharmaceutical University
基金
广东省医学科研基金资助项目(No.B2005104)
广东省中医药局课题资助项目(No.1060044)~~
