摘要
为了建立测定食品中的叶黄素含量的可靠方法,采用高效液相色谱法进行了测定。使用硅胶色谱柱(4.6mm×250mm,5μm),以环已烷:乙酸乙酯=75:25为流动相,检测波长445nm。该方法最低检出量1.0mg/kg,RSD为1.2%,加标回收率为93.8%~105.6%,RSD为3.1%~4.1%。该方法简便、准确,有良好的重现性,技术参数指标符合食品理化分析的要求。
A HPLC method was established for the quantitative determination of lutein in foods. The HPLC used Rx-sil column, a mixture of cyclohexane : ethyl acetate = 75 : 25 as mobile phase, and UV detection at 445nm. The detection limit was 1.0 mg/kg,The RSD was 1.2% ;the recovery of added lutein was 93.8 % - 105.6%, the RSD was 3.1% -4.1%. This method is simple, accurate and reproducible.
出处
《粮油食品科技》
2007年第1期60-62,共3页
Science and Technology of Cereals,Oils and Foods
关键词
高效液相色谱法
叶黄素
定量检测
:high performance liquid chromatography
lutein
quantitative determination