摘要
为了建立测定食品中的叶黄素含量的可靠方法，采用高效液相色谱法进行了测定。使用硅胶色谱柱（4．6mm×250mm，5μm），以环已烷：乙酸乙酯=75：25为流动相，检测波长445nm。该方法最低检出量1．0mg／kg，RSD为1．2％，加标回收率为93．8％～105．6％，RSD为3．1％～4．1％。该方法简便、准确，有良好的重现性，技术参数指标符合食品理化分析的要求。
A HPLC method was established for the quantitative determination of lutein in foods. The HPLC used Rx-sil column, a mixture of cyclohexane ： ethyl acetate = 75 ： 25 as mobile phase, and UV detection at 445nm. The detection limit was 1.0 mg/kg,The RSD was 1.2% ;the recovery of added lutein was 93.8 % - 105.6%, the RSD was 3.1% -4.1%. This method is simple, accurate and reproducible.
出处
《粮油食品科技》
2007年第1期60-62,共3页
Science and Technology of Cereals,Oils and Foods
关键词
高效液相色谱法
叶黄素
定量检测
：high performance liquid chromatography
lutein
quantitative determination
