糖尿病患者的非糖尿病一级亲属血浆降钙素基因相关肽水平的研究

Level of calcitonin-gene related peptide in nondiabets first-degree relatives of type 2 diabetes

目的观察降钙素基因相关肽(CGRP)与内皮素(ET)在2型糖尿病(T2DM)患者及非糖尿病的T2DM一级亲属(FDRs)中的变化,并探讨其在T2DM发生中的作用。方法采用放免法分别测定28例正常人,35例T2DM患者(T2DM组A,T2DM组B)及30例非糖尿病的T2DM一级亲属血浆ET,CGRP含量。结果T2DM A、B组患者血浆ET值较正常对照组显著增高,(64.67±7.92)ng/L,(79.91±8.33)ng/L,vs(5.07±6.24)ng/L(P<0.01),CGRP值显著降低,(144.18±24.16)ng/L,(121.32±23.29)ng/L vs(180.36±21.73)ng/L(P<0.01),FDRs组血浆ET值较正常对照组显著增高,(60.79±7.50)ng/L vs(50.07±6.24)ng/L(P<0.05),CGRP值显著降低,(157.87±18.22)ng/L vs(180.36±21.73)ng/L(P<0.05),有糖尿病家族史比无糖尿病家族史者ET显著升高(P<0.05)、CGRP显著降低(P<0.05);CGRP与胰岛素抵抗指数(HOMA-IR)呈负相关(r=-0.19,P=0.049),ET与HOMA-IR呈正相关(r=0.286,P=0.029)。结论T2DM患者及非糖尿病的T2DM一级亲属ET增高,CGRP降低,提示遗传因素可能参与了糖尿病的发病机制。

Objective To observe the changes of plasma endothelin（ET） and calcitonin-gene related peptide （CGRP） levels in type 2 diabetes（T2DM） and nondiabetes first-degree relatives（FDRs） of T2DM. Methods The concentrations of plasma ET and CGRP were measured by means of radioimmunoassay in 28 normal subjects, 35 cases of diabetic patients（T2DM A,T2DM B） and 30 cases of nondiabetes first-degree relatives（FDRs） of T2DM. Results The concentration of ET in T2DM A, T2DM B and FDRs group was markedly higher than that in control group, （64.67±7.92） ng/L,（79.91±8.33） ng/L vs （5.07±6.24）ng/L（ P〈0.01）. The concentration of calcitonin-gene related peptide（CGRP） in T2DM A, T2DM B and FDRs group was markedly lower than that in control group, （144.18 ± 24.16） ng/L,（121.32±23.29） ng/L vs （180.36±21.73） ng/L（ P〈0.01）. The concentration of ET in FDRs group was ignificantly higher than that in control group, （60.79±7.50） ng/L vs （50.07±6.24） ng/L（ P 〈0.05） ,but the concentration of CGRP was significanthy lower, （157.87±18.22） ng/L vs （180.36±21.73） ng/L（ P〈0.05）. CGRP and ET were correlated with HOMA-IR（ r = 0.19, P 0. 049 ; r = 0. 286, P = 0. 029）. Conclusion Plasma ET increased and CGRP decreased in T2DM and FDRs of T2DM, and there was relationship between generation and T2DM.