摘要
目的:探讨TGF-β及其伙伴分子Smad4在胃癌组织中的表达。方法:利用逆转录(RT)-PCR技术测定胃癌组织TGF-β mRNA和Smad4 mRNA水平,并分析两者与胃癌患者各项临床病理指标间的关系。结果:Smad4 mRNA在胃癌组织中的阳性表达率明显下降。TGF-β和Smad4均与胃癌组织的分化程度、肿瘤大小、浸润深度、淋巴转移和TNM分期有关。Smad4 mRNA在胃癌组织分化程度高、肿瘤<5cm、浸润深度浅、无淋巴转移、TNM分期较早的患者中阳性表达率高。TGF-β mRNA在胃癌组织中的阳性表达率明显升高。TGF-β mRNA在胃癌组织分化程度高、肿瘤<5cm、浸润深度浅、无淋巴转移、TNM分期较早的患者中阳性表达率低。胃癌组织中TGF-β和Smad4表达间呈负相关。结论:TGF-β和Smad4共同调节胃癌的发生、发展及其生物学行为。
Objectives： To explore the correlation of Smad4 mRNA and TGF-β mRNA in gas-tric carcinoma. Methods： The presence of Smad4 mRNA and TGF-β mRNA was measured by re-verse transcriptase polymerase chain reaction （RT-PCR） from 61 gastric carcinomas and 39 normal controls. Results： The positive percentages of Smad4 mRNA in gastric carcinoma were lower than those of normal controls. The positive percentages of TGF-β mRNA in gastric carcinoma were high-er than those of normal controls. The expressions of Smad4 mRNA and TGF-β mRNA were all re-lated to histological differentiation, location, tumor size, infiltration, lymph node metastasis and TNM stage. Negative correlation occurred in Smad4 mRNA and TGF-β mRNA. Conclusion： TGF-β and Smad4 may be associated with the carcinogenesis, biological behaviors and prognosis of gastric carcinoma.
出处
《中国现代普通外科进展》
CAS
2007年第3期194-198,共5页
Chinese Journal of Current Advances in General Surgery
基金
山东省科技厅资助项目(2003-23)
关键词
胃肿瘤
逆转录-聚合酶链反应
转化生长因子
β
SMAD4基因
Gastric neoplasms
Reverse transcriptase polymerase chain reaction
Transforminggrowth factor β
Smad4 gene
