基质金属蛋白酶及抑制因子与腹主动脉瘤临床病理特点的相关性研究

The study of relationship between matrix metalloproteinase and tissue inhibitor of metalloproteinase and the clinical pathology of human abdominal aortic aneurysm

目的:研究基质金属酶(MMP)-2、MMP-9及抑制因子TIMP-1在腹主动脉瘤中的表达及与临床病理特征之间的关系。方法:应用免疫组化PV-9000通用型二步法对70例腹主动脉瘤和15例正常腹主动脉标本中的MMP-2、MMP-9及TIMP-1表达进行检测。结果:腹主动脉瘤组织中MMP-2和MMP-9蛋白表达阳性率明显高于正常腹主动脉组织,TIMP-1蛋白表达阳性率和正常腹主动脉没有统计学差异,(χ2=0.103,P=0.991);MMP-2蛋白的表达与腹主动脉瘤的直径呈负相关(χ2=13.785,P=0.032),MMP-9蛋白的表达与患者临床症状,腹主动脉瘤直径、破裂有相关性,(P<0.05),TIMP-1蛋白表达阳性率与临床病理特征无相关性(χ2=0.103,P=0.991)。结论:腹主动脉瘤组织中MMP高表达和TIMP的相对弱表达在腹主动脉瘤发生、发展过程中起重要作用,MMP-9可以预测腹主动脉瘤的自然病程从而作为腹主动脉瘤手术治疗的指征之一。

Objectives： To explore the expression of matrix metalloproteinase MMP-2, MMP-9 and tissue inhibitor of metalloproteinase TIMP-1 in human abdominal aortic aneurysm （AAA）. Methods： The MMP-2, MMP-9 and TIMP-1 were measured by the immunohistochemical two-step methods of PV-9000 in seventy specimens of AAAs and 15 normal abdominal aorta tis-sues. Results： The positive rate of MMP-2 and MMP-9 in AAA were significant highly than those in normal abdominal aorta tissues. The expression of MMP-2 and the diameter of AAA had signifi-cant negative-correlation. The expression of MMP-9 had significant correlation with the diameter of AAA, rupture and symptom. Significant difference was not found between the positive rate of TIMP-1 in AAA and normal abdominal aorta. The expression of TIMP-1 had no correlation with clini-cal pathological features of AAA. Conclusion： The study suggests the principal metalloproteinase in small aneurysms was MMP-2 and MMP-9 was involved in aneurysms expansion at a later stage.