摘要
脂肪酸值是粮食储存品质的重要指标,本研究采用比色法来测定粮食脂肪酸值。本方法使用石油醚提取粮食脂肪酸,利用异辛烷,双-(2-基己基)磺基丁二酸钠和pH=9.0的酚红Tris/HCl溶液形成的透明反胶团体系进行比色分析。通过比色法和国标法相比较测定了玉米粉、糙米和小麦三种样品的脂肪酸值;结果表明:比色法和国标法两者之间相对误差分别为18.65%,11.24%和6.61%。比色法的精密度较高,标准偏差相对值在2.0%和5.3%之间。比色法具有快速灵敏、样品和试剂用量少、测定结果客观、便于大批量样品测定等优点。
Fatty acid value is an important quality index of stored grain. The determination of fatty acid value in grain by coiorimetric method was proposed in this study. Fatty acid in grain was extracted with petroleum ether. The transparent reverse micellar solutions made of isooctane, his （2 - ethylhexyl） sodium sulfosuccinate and phenol red - tris/HCI solution were used for colorimetric analysis of free fatty acids. Fatty acid values of samples of corn flour, coarse rice and wheat were determined by the colorimetric method and the national standard method. Results showed that the relative errorw between two methods were 18. 6596,11. 2496 and 6. 6196 respectively.
The colorimetric method was precise with standard deviation varied between 2.0 % and 5.3 %. There are some advantages of the colorimetric method. It is fast, sensitive. It needs small amount of sample and consumes less organic solvent. It is ideal for analysis of large number of samples.
出处
《粮食储藏》
2007年第3期43-45,共3页
Grain Storage
关键词
比色法
粮食
脂肪酸值
反胶团
colorimetric method, grain, fatty acid value, reverse micelle
