HLA-DR13基因与丙型肝炎病毒感染外周血单个核细胞的关系

Association between HLA-DR13 and Peripheral Blood Mononuclear Cell(PBMC) in HCV Infection

目的:探讨人类白细胞抗原(HLA)中的DR13基因对慢性丙型肝炎患者中丙型肝炎病毒(HCV)感染外周血单个核细胞(PBMC)的影响。方法:选择75例已经确诊为慢性丙型肝炎患者,采用PCR-SSP方法检测HLA-DR13基因,用密度梯度离心法分离收集PBMC,按是否携带HLA-DR13基因分为两组,观察HLA-DR13基因与HCV感染PBMC的关系。结果:本组携带HLA-DR13基因者17例,PBMC阳性4例(23.5%),未携带HLA-DR13基因者58例,PBMC阳性36例(62.1%),采用四格表χ2检验,两组差异有统计学意义(χ2=7.85,P<0.01)。结论:HLA-DR13基因可能与HCV感染PBMC有关。

Objective：To study the effect of HLA-DR13 on PBMC in HCV infection. Methods：Genomic DNA was analyzed by pol- ymerase chain reaction/sequence specific primer（PCR-SSP） technique,and the PCR products were purified and sequenced. RTPCR was applied to detect HCV RNA in PBMC. Results：Of 75 chronic hepatitis C patients, HLA-DR13 was detected in 17 patients in which 4 （23.5%） patients with PBMC-HCV RNA positive. HLA-DR13 was negative in 58 patients in which 36 （62.1% ） patients with PBMC-HCV RNA positive, there was significant difference（ X^2 = 7.85, P 〈 0.01 ）. Conclusion： HLA-DR13 maybe have some influence on PBMC in HCV infection.