黄芪注射液对大鼠局灶性脑缺血的抗氧化作用

Anti-oxidation Effects of Astragalus Injection on Cerebral Ischemia-reperfusion in Rats

目的探讨黄芪注射液(AI)对大鼠局灶性脑缺血-再灌注后脑组织中SOD、MDA含量的影响。方法:雄性SD大鼠24只,随机分为假手术组、缺血组、AI剂量组,每组8只。采用尼龙线大鼠大脑中动脉栓塞法造成大鼠脑缺血-再灌注模型。用黄嘌呤氧化酶法和硫代巴比妥酸法测定脑组织丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果AI能明显降低脑组织MDA含量,增加脑组织SOD活性(P<0.01)。结论AI通过清除氧自由基而发挥对脑缺血-再灌注后脑组织的保护作用。

Objective To study the effects of Astragalus injection （AI） on the level of SOD and MDA following focal cerebral ischemia-reperfusion in rats. Methods 24 male Sprague-Dawley rats were randomly divided into sham group, ischemia group, AI dose group. There were 8 animals in each group. The animal models cerebral ischemia-reperfusion by middle cerebral artery occlusion （MCAO） were established in rats. At 24h after MCAO for 2h, the activity of SOD and the content of MDA were measured respectively by xanthine oxidase method and thiobarbituric acid（TBA） method. Results Com- pared with ischemia group, AI could significantly increase the activity of SOD and decrease the content of MDA in brain tissue （P〈0. 01）. Conclusion AI has protective effects on cerebral ischemic injury by enhancing the ability of free radicals scavenging.