大鼠孤束核微量注射乙酰胆碱对电针抗大鼠应激性胃黏膜损伤的影响

The Effects of Microinjection of Acetylcholine into Nucleus Tractus Salitarious on Electroacupuncture Against Stress Mucosa Injury in Rats

目的观察孤束核内乙酰胆碱(Ach)对电针(EA)抗大鼠应激性胃黏膜损伤的影响。方法84只雄性SD大鼠,随机分为正常组、应激组、EA+应激组、NS+EA+应激组、Ach+EA+应激组、阿托品+EA+应激组。采用束缚-浸水制备大鼠应激性胃溃疡模型,观察孤束核微量注射Ach、M受体阻断剂阿托品,大鼠胃黏膜溃疡指数(UI)、超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量的变化。结果与单纯应激组比较,EA+应激组胃黏膜UI、MDA含量降低,SOD活性增高。Ach+EA+应激组上述作用更明显。孤束核给予阿托品处理,可减弱电针抗胃黏膜损伤的作用。结论孤束核内Ach参与了电针对大鼠应激性胃黏膜损伤保护作用过程。

Objective To observe the effects of acetylcholine （Ach）in nucleus tractus solitarious on electroacupuncture （EA） against gastric stress ulcer in rats. Methods A total of 84 male SD rats were randomly divided into normal control group, stress group, EA＋ stress group, NS＋ EA＋ stress group,Ach＋ EA＋ stress group and atropine＋ EA＋ stress group. The gastric stress ulcers models were established by restrain and water immersion. The nucleus tractus solitarious was injected by the Brain Stereota-xic Frame. The variation of the gastric ulcer index（UI） and superoxidatase （SOD） ac- tivity and malondialdehyde（h4DA） level were observed in rats that were separately microinjected Ach and M receptor antagonist-atropine in the nucleus of tractus solitarius. Results Compared with stress group, EA＋stress group showed that the UI and MDA level were decreased but SOD activity was in- creased. Pretreatment with Ach into nucleus tractus solitarious enhanced the effects of electroacupunc- ture against stress mucosa injury,by contrast, pretreatment with atropine significantly attenuated the same effects. Conclusion The Ach in the nucleus of tractus solitarius involve in the protective effects process of the stressing gastric mucosa lesien by EA in rats.