糖尿病肾病大鼠肾脏cbfa1表达与钙化的关系

The Relationship Between Renal Calcification and Expression of cbfa1 in Rat Diabetic Nephropathy

目的:探讨糖尿病肾病大鼠肾脏钙化与骨分化核心结合因子1(cbfa1)表达的关系。方法:用链脲佐菌素诱导糖尿病肾病大鼠模型,并以正常大鼠作为对照组,于第3、6月末分别采用Von Kossa染色观察肾脏钙盐沉积、肾脏免疫组化、RT-PCR、Wester Blot法观察肾脏cbfa1的表达水平。结果:糖尿病肾病大鼠肾脏有cbfa1的表达,且与钙盐沉积程度有关。正常大鼠肾脏没有发现cbfa1的表达。结论:糖尿病肾病晚期时肾脏出现钙盐沉积,肾脏cbfa1的表达水平与钙盐沉积程度呈正相关。

To explore the relationship between renal calcification and the expression of osteoblast- specific transcription factor- core binding factor alpha 1 （cbfal） in rats with diabetic nephropathy. Methods： At the end of 3rd and 6th month, renal calcification and cbfal expression were examined in rats with STZ - induced diabetic nephropathy. Meanwhile, normal rats were used as control groups. Blood, urine and kidney samples were taken at 3rd and 6th month. Expression of cbfal was detected by immunohistoehemistry, reverse transcription polymersase china reaction （RT- PCR） and Western blotting at 3rd and 6th month. Renal calcification （calcium deposition） was evaluated by Von Kossa staining. Results： There was expression of cbfal in the kidney with diabetic nephropathy. There was a positive correlation between the expression of cbfal and renal calcium deposition. There was no expression of cbfal in normal rats. Conclusion： There are calcium depositions in the kidney of advanced diabetic nephropathy in rats, which is associated with cbfal expression.