铁叶绿酸钠对正常小鼠祖细胞集落和实验性贫血的影响

Effect of sodium iron chlorophyllin (SIC) on CFU E and CFU GM yields of normal and anemic animal models

目的：探讨铁叶绿酸钠（ＳＩＣ）对骨髓造血细胞的作用和对鼠实验性贫血的疗效。方法：用体外小鼠骨髓细胞培养技术及失血性贫血大鼠与溶血性贫血小鼠模型，观察ＳＩＣ对骨髓造血祖细胞增殖及实验性贫血的影响。结果和结论：①ＳＩＣ能明显促进正常小鼠骨髓红系祖细胞（ＣＦＵ－Ｅ）和粒－单核细胞系祖细胞（ＣＦＵ－ＧＭ）的增殖反应；②ＳＩＣ能提高正常小鼠外周血网织红细胞的百分比；③对乙酰苯肼所致小鼠溶血性贫血，ＳＩＣ可减轻外周血红细胞和血红蛋白的降低程度；④ＳＩＣ可促进失血性贫血大鼠红细胞、血红蛋白和网织红细胞恢复正常，并提高血清铁水平和转铁蛋白饱和度。上述结果为ＳＩＣ用于治疗贫血提供了药理学基础。

Objective:To observe the effect of sodium iron chlorophyllin (SIC) on hematopoiesis and its therapeutic efficacy in anemic mice.Methods:The effects of SIC on CFU E and CFU GM yields of normal and anemic animal models were studied.Results and Conclusions:①SIC significantly enhanced the CFU E and CFU GM yields of mouse bone marrow.② SIC also increased the percentage of reticulocyte in mouse.③In hemolytic anemia mice induced by i.p N acetphenylhydrazine,SIC markedly prevented the decrease of RBC and Hb.④In hemorrhagic anemia rats induced by bleedletting,SIC accelerated the normalization of RBC ,Hb and elevated the serum iron level and transferrin saturation.