摘要
目的:优选固骨缓释胶囊中淫羊藿、骨碎补有效部位提取和分离纯化工艺的最佳条件。方法:以淫羊藿苷、柚皮苷、干膏收率(%)为指标,采用正交试验法考察最佳提取和分离纯化工艺,用HPLC法测定目标成分含量。结果:最佳渗漉工艺为过3号筛的粗粉以80%乙醇浸泡12h,流速为5mL·min^(-1)(以1kg药材计),渗漉量为6倍量;最佳精制工艺为加入1.5%活性炭,调pH值为1~2,加入8%食盐。结论:该最佳纯化工艺条件得到的结果稳定,活性成分含量高,可以用于工业生产。
OBJECTIVE: To optimize the conditions for the extraction and purification of the active components of Epimedium Herb and Drynaria in Gugu sustained-release capsules. METHODS: The contents of Icariin, Naringin, and yield of dry extract(%) were taken as indexes; the optimum conditions for the extraction and purification were investigated by orthogonal experiment, and the contents of samples were determined by HPLC. RESULTS: The optimum percolating process was as follows: soaking the crude herb powder that meshed through NO. 3 sifter in 6-fold 80% ethanol for 12 hours; the flow velocity was 5mL·min^-1 (1kg medical material was computed) . The optimum purification condition was as follows: 1.5% active carbon was added; adjusting pH value to 1-2, and 8% NaCl was added. CONCLUSION: This optimized purification technology was proved to be of stable results and high level of active components, and it can be used for the practical production.
出处
《中国药房》
CAS
CSCD
北大核心
2007年第21期1633-1635,共3页
China Pharmacy
关键词
固骨缓释胶囊
正交试验
提取
分离纯化
淫羊藿
骨碎补
有效部位
Gugu sustained-release capsule
Orthogonal experiment
Extraction
Extraction and purification
Epimedium Herb
Drynaria
Active part