正交试验优选乙醇温热湿润灭菌的最佳条件

Optimization of Methanol Lukewarm Moist Sterilization Conditions by Orthogonal Test

目的:优选中药泛制丸卫生学控制指标中乙醇温热湿润灭菌的最佳条件。方法:采用正交试验法,以乙醇的比例、温度、湿润时间为3因素,对自制素丸样品在不同水平下检测灭菌效果。结果:乙醇温热湿润灭菌的最佳条件为加入75%乙醇的比例为1.67%(mL·g^(-1)),温度为50～60℃,湿润时间为1h。结论:该方法简便、实用、有效,可用于中药泛制丸的卫生学控制。

OBJECTIVE： To optimize methanol lukewarm moist sterilization conditions in the hygienic control of Chinese processing pills. METHODS： The optimization was performed by orthogonal test with ratio, temperature and moisture time of methanol as 3 factors. The sterilization efficacy of the self-made sample pills at different levels was detected. RESULTS： The optimum lukewarm moist sterilization conditions were as follows： the ratio, temperature, and moisture time of 75% methanol were 1.67% （mL·g^-1）, 50-60℃, and lh, respectively. CONCLUSION： The method was proved to be simple, practical and effective, and it can be used for the hygienic control of Chinese processing pills,