肺癌染色体遗传标记 2q^- 在癌变和发展中的意义

Chromosome Marker——2q - during carcinogenesis and development of human lung cancer

目的研究肺癌发生过程中的细胞遗传学改变，探讨肺癌发生的遗传学机理。方法应用荧光原位杂交（ＦＩＳＨ）技术，对１例已永生化并趋于恶性的人支气管上皮细胞系Ｍ，以及１２例临床原发非小细胞肺癌（ＮＳＣＬＣ）标本中２号和３号染色体改变进行了研究。结果２ｑ－和３ｐ－高频发生。Ｍ细胞系的晚期代数ＭＰ７１及检测的７例ＮＳＣＬＣ标本中的３例有２ｑ－，Ｍ细胞系及１２例标本中的５例有３ｐ－。结论２ｑ－也是肺癌中高发的细胞遗传学改变。可能在２ｑ上存在一个或几个肿瘤抑制基因，其丢失可导致ＮＳＣＬＣ的发生和发展。

Objective To study the cytogengtic alterations during the development of human lung cancer and to clarify the genetic basis of lung cancer. Methods Using Fluorescence in situ Hybridization FISH, we have studied the chromosomal alterations of chromosome 2 and 3 in human bronchial epithelial cell line M, and 12 primary non small cell lung cancer (NSCLC) specimens. Results 2q - was found in the later passage of human bronchial epithelial cell line M, and 3 out of 7 primary NSCLC specimens tested. 3p - being noted in five cases of 12 primary NSCLC patients examined was the first cytogenetic discovery and the most prominent abnormality in lung cancer. 2q - was rarely reported in human lung cancer before. But loss of heterozygosity by RFLP analysis for 2q had been reported in lung cancer.Conclusion 2q - is also a common chromosome abnormality in human lung cancer. There would also be one or more putative tumor suppressor gene(s) on the long arm of chromosome 2. Loss of the gene(s) may contribute to the carcinogenesis of human non small cell lung cancer.