枸杞籽油抗动脉粥样硬化的功效强度及作用机制的实验研究

Experimental Study of Anti-atherosclerosis Potency by Lycium Seed Oil and Its Possible Mechanism

目的:研究枸杞籽油抗动脉粥样硬化的功效强度及涉及的可能机制。方法:高脂膳食制备家兔动脉粥样硬化实验模型,动态观测其血浆TC、TG、LDL-C、HDL-C的变化;8周后,测定血清SOD、GSH-Px、T-AOC活性和MDA的含量;制作石蜡切片进行病理学检测;采用RT-PCR和免疫组化分析NFκ-Β、TNF-α在血管中的表达。结果:枸杞籽油可增加实验家兔血浆HDL-C含量,降低血浆TC、TG、LDL-C含量,增强血清SOD、GSH-PX、T-AOC活性,降低血清MDA、TNF-α的含量;降低NFκ-Β、TNF-α分子在血管中的表达,减少脂质斑块的形成。其效应强度与脂必妥相当。结论:枸杞籽油具有显著的抗动脉粥样硬化效应。其机制涉及降血脂、抗氧化,抑制动脉粥样硬化病灶中NFκ-Β的通路,降低血清中炎性分子TNF-α的含量。

Objective: To study the effect of anti-atherosclerosis of Lycium Seed Oil(Lso) and its possible mechanism.Methods: The rabbit atherosclerosis model was established by high fat diet,and the TC、TG、LDL-C、HDL-C levels in plasma were examined dynamically.The SOD、GSH-PX,T-AOC activities and the MDA levels in serum were monitored after 8 week's high fat diet.Aorta samples were observed for atherosclerotic extent,and NF-κΒ、TNF-α were assessed by immuno-histochemical method.The lovastatin was set up as a positive control.Results: contents of HDL-C obviously increased in Plasrna of low and high dosage groups and TC、 TG、LDL-C levels significantly decreased compared with control group.The SOD、GSH-PX,T-AOC activities up-regulated while the NF-κΒ、MDA and NF-α levels decreased in Lycium Seed Oil groups compared with control group.Aortic atherosclerotic extent and area in low dosage and high dosage LSO groups were absolutely smaller than that in high fat diet group.The anti-atherosclerosis potency of Lycium Seed Oil was similar with that of lovastatin.Conclusion: Lycium Seed Oil has potent anti-atherosclerosis effects and its anti-atherosclerosis potency was similar with The lovastatin.The possible mechanism involve the decreasing of plasma lipids,anti-peroxidation,inhibiting the activation of NF-κΒ and down-regulating the inflammation cytokines of TNF-α.