珍珠梅提取物抑制肝癌HepG-2细胞增殖的实验研究

Inhibition of Sorbaria sorbifolia on Proliferarion of Hepatoma HepG-2 Cell Line

目的:观察珍珠梅提取物对肝癌HepG-2细胞增殖的影响,探讨其作用机制。方法:采用MTT比色法观察珍珠梅提取物抑制肝癌HepG-2细胞增殖;采用流式细胞仪检测其对HepG-2细胞周期分布的影响,同时结合倒置显微镜、HE染色、透射电镜及免疫细胞化学方法检测凋亡相关基因bcl-2、p53的蛋白表达,观察其诱导肝癌HepG-2细胞凋亡的作用。结果:珍珠梅提取物可抑制肝癌HepG-2细胞增殖,其作用具有时间和浓度依赖性;珍珠梅提取物可诱导细胞凋亡;同时改变细胞周期分布,多数细胞阻滞于G2/M期,与对照组比较,细胞凋亡率差异有显著性(P<0.01);用药前后凋亡相关基因bcl-2、p53蛋白表达均有显著性改变(P<0.01)。结论:珍珠梅提取物可诱导肝癌HepG-2细胞凋亡,改变细胞周期分布,从而抑制细胞增殖。

Objective： To investigate the inhibition of Sorbaria sorbifolia on proliferation hepatoma HepG-2 cells, and to explore the antineoplastic mechanism of Sorbaria sorbifolia. Methods ： MTr assay was used to examine the effect of Sorbaria sorbifolia on the proliferation of HepG-2 cell and flow cytometry was used for the cell cycle distribution. Apoptosis of HepG-2 cell was investigated further by means of inverted microscope, HE staining, transmission electron microscopy and expression of apoptosis-assoeiated gene bcl-2 andp53 were determined by immunohistochemical method. Results： Sorbaria sorbifolia inhibited the proliferation of HepG-2 cell and the effects were in a time-and-concentration dependent manner. Sorbaria sorbifolia could also induce apoptosis, the G2/M phase was arrested and the ratio of apoptosis was significantly difference with that of control group, respectively. The expressions of apoptosis-associated gene bcl-2 and p53 had changed significantly, respectively. Conclusion： Sorbaria sorbifolia inhibits proliferation of HepG-2 cells via induction of apoptosis and cell cycle arrest.