铁牛七生物碱提取工艺研究

Study on the Optimum Extracting Conditions of Total Alkaloids and Aconitine in Aconitum szechenyianum Gay.

目的:优选铁牛七中生物碱的提取工艺。方法:采用L16(45)正交设计并结合单因素轮换法,以酸性染料比色法测定总生物碱含量,以高效液相色谱法测定乌头碱含量,对铁牛七中生物碱的提取条件进行优化。结果:总碱和乌头碱具有不同的最佳提取条件。采用6倍量酸性乙醇溶液(无水乙醇∶pH 3.0 HAc=85∶15,v/v)回流提取1 h时总生物碱提取量最高,3次提取基本可将其完全提出,测得总生物碱含量为0.980%;采用4倍量酸性乙醇溶液(无水乙醇∶pH 3.0 HAc=15∶85,v/v)回流提取0.5 h时乌头碱提取量最高,3次提取基本可将其完全提出,测得乌头碱含量为0.109%。结论:实验证明该提取工艺科学、合理。

Objective： To optimize the conditions for extractions of total alkaloids and aconitine from Aconitum. szechenyianum Gay. Methods： Using L16 （4^5） orthogonal test and univariant methods, the exrtaetion conditions of total alkaloids aeonitine in Aconitum szechenyianum Gay. were optimized under the guidance of the content determination of total alkaloids with bromophemol blue eolorimetry and aeonitine with HPLC method, respectively. Results ： The obtained optimum condition of total alkaloids was that the material was refluxed in 6 times（m/v） acidic alcohol solution（ alcohol： pH 3.0 HAe = 85：15, v/v）for 1 h with 3 times. The condition of aeonitine was that the material was refluxed in 4 times （m/v） acidic alcohol solution （alcohol： pH 3. p HAe = 15： 85, v/v）for 0. 5 h with 3 times. Contents of total alkaloids and aeonitine in Aconitum. szechenyianum Gay. were 0. 980% and 0. 109% , respectively. Conclusion： Through the test and verify, the optimum extraction methods of total alkaloids and aeonitine are rational.