天麻多糖水提及脱蛋白工艺优化

Optimization of Extraction Parameters and De-protein Process for Gastrodia elata.Polysaccharides

目的:摸索天麻多糖水提和脱蛋白工艺条件,为天麻多糖提取纯化的工业化提供理论依据。方法:采用正交实验研究了温度、物料比、时间对天麻多糖(GPS)提取工艺的影响;对比了两种不同的脱蛋白方法(Sevag法和酶-Sevag法)在天麻多糖纯化工艺中的应用。结果:水提最佳工艺为物料比1∶40,浸提温度120℃,浸提时间3 h。酶-Sevag法相对于Sevag法除蛋白效果好,且多糖得率高。

Objective： Grope for Optimization of Extraction Parameters and De-protein Process for Gastrodia elata Bl, Polysaeeharides （GPS）. Provide the theoretical basis for the industrialization production of GPS. Methods ： The effects of extracting temperature, ration between Gastrodia elata Bl. and water, heating time on extraction rate of GPS were studied with orthogonal experimental design. Compared the application of the two de-protein process measures （ Scrag and Enzyme-Sevag） in purification technics for Gastrodia data Bl. Results ： The optimum parameters were： extraction 3h at 120℃ with 1 ： 40 ratio of Gastrodia elata B1. to water. The de-protein efficiency of Enzyme-Sevag is better than Sevag.