重楼总皂苷提取工艺研究

Studies on the Extraction Process of Total Saponins from Paris polyphylla Smith

目的:优选重楼总皂苷提取工艺。方法:以总皂苷得率为评价指标,采用单因素试验和正交试验优选最佳提取工艺。结果:优化后重楼总皂苷的提取工艺为:70%乙醇提取两次,第一次用10倍量提取2 h,第二次用8倍量提取1.5 h;按优选出的工艺进行提取,总皂苷得率为4.24%,重楼总苷Ⅰ、Ⅱ总提取率为93.28%。结论:验证试验结果表明,优选出的工艺稳定、合理、可行。

Objective： To optimize the extraction process of total saopnins from Paris polyphylla Smith. Methods ： The single factor test and orthogonal experiment were used to determine the optimum extraction process. Results： The optimum extraction process was obtained as follows： the plant materials were extracted with 70% ethanol twice, respectively with 10BV for 2 hours and then with 8BV the solvents for 1.5 hours. The yield of total saponins could be up to 4. 24% and the total extraction rate of Paris polyphylla Ⅰ and Paris polyphylla Ⅱ was 93.28%. Conclusion： The optimum process obtained is steady, reasonable and feasible.