缺锌并补充睾酮对大鼠睾酮合成和骨骼肌影响的研究

The Effects of Zinc Deficiency and Testosterone Supplement on Testosterone Synthesis and Skeletal Muscle of Rats

观察了大鼠缺锌和缺锌后补锌以及给这些大鼠补充睾酮对睾酮合成和骨骼肌的影响。结果显示：缺锌引起大鼠体重、睾丸重量、睾丸和血清睾酮含量、趾长伸肌重量、股四头肌深层肌总ＲＮＡ含量的明显降低。补充睾酮，不仅对这些指标的降低无改善作用，反而抑制自身睾酮的合成，进一步引起睾丸的萎缩。缺锌和补充睾酮对骨骼肌肌动蛋白、ＤＮＡ、总蛋白的含量影响不大。缺锌大鼠经过补锌，上述各项指标就能恢复或基本恢复。实验表明，锌缺乏时骨骼肌合成代谢受阻，与睾酮的减少没有关系。

The effects of zinc deficiency and testosterone(T) supplement on T synthesis and skeletal muscle were investigated in zinc deficient and zinc replenished rats. The results showed that there were significantly lower weights of body, testes and musculus extensor digitorum longus, lower contents of T in testes and serum and total RNA in the profundus muscularis of quadriceps femoris of zinc deficient rats. T supplement had no favourable effects on the decreased indices mentioned above. On the contrary, T supplement inhibited the endogenous synthesis of T and caused further atrophy of testes in zinc deficient rats. The concentrations of actins, DNA and total protein in skeletal muscle were not influenced markedly by zinc deficiency and/or T supplement. It suggested that the decrease of anabolism in skeletal muscle is not related to the low level of T in the rats under the condition of zinc deficiency.