保心丸对实验性动脉粥样硬化家兔血脂、载脂蛋白、过氧化脂质动态变化的影响

Effects of Baoxin Pills on the Dynamic Changes of Blood Lipid, Apolipoprotein and Lipid Peroxidein Rabbits with Experimental Atherosclerosis

用日本大耳白家兔造成动脉粥样硬化 (AS)模型 ,防治组同时给予保心丸 8g/kg ,另设诺衡防治组作对照 ,在实验的不同阶段动态观察血脂、载脂蛋白、过氧化脂质的含量。实验结果显示 :保心丸能明显降低血清TC、LDL c、AI(血管硬化指数 )、apoB10 0 及TG ,升高HDL c/LDL c、HDL c/TC比值 ,明显降低血浆LPO含量 ,而对血浆红细胞SOD活性无明显影响。病理观察显示 :保心丸能保护血管内皮细胞 ,抑制平滑肌细胞 (SMC)增殖、迁移 ,减少血管中膜弹力纤维和胶原纤维的形成 ,从而明显减小粥样斑块面积。保心丸调脂作用的关键是降低LDL c ,同时降低apoB10 0 、LPO也参与了抗AS过程。诺衡以降低血清TG、升高apoA1为显著 。

Japan white rabbits were fed on l gram of cholesterol and 4 grams of lard per day for 2 months to induce atherosclerosis, Baoxin pills group was simultaneously given Baoxin pills 8 grams per kilogram of body weight, and the control group was given Gemfibrozil 80 milligrams per kilogram of body weight. The contents of blood lipid, apolipoprotein and lipid peroxide were determined in each phase of the experiment and rabbit aortas were stained with hematoxylin, oil red 0 and by a special stain method. The results showed that Baoxin pills could significantly lower serum TC, LDL c, TG, atherogenic index and apolipoprotein B 100 , increase the rates of HDL c/TC, and HDL c/LDL c, and significantly lower plasma LPO content. Pathological observation showed that Baoxin pills could protect vascular endothelial cells, inhibit proliferation and migration of smooth muscle cells, and reduce the formation of elastic fibers and collageneous fibers in media, which therefore evidently decreased atherosclerotic plaque area. The key of Baoxin pills in regulating blood lipid metabolism was lowering LDL c. The lowering of LDL c and LPO contents caused the decrease of oxidized LDL and MDA LDL, and therefore inhibited the development of atherosclerosis. Gemfibrozil could significantly decrease TC and increase apolipoprotein A 1, but had no any influences on other biological substances.