家蚕表达的重组人巨噬细胞集落刺激因子促进放射性损伤小鼠血细胞的恢复

Recombinant human macrophage colony stimulating factor expressed in silkworm accelerates hematopoietic recovery of radiolesion mice

目的：研究重组人巨噬细胞集落刺激因子（ｒｈＭ－ＣＳＦ）对骨髓造血功能恢复的影响。方法：以亚致死剂量６０Ｃｏγ射线照射ＢＡＬＢ／ｃ小鼠，造成放射性损伤，继以家蚕表达的ｒｈＭ－ＣＳＦ连续７天给每只小鼠腹腔注射进行治疗。结果：照射后第１５和２０天，治疗组小鼠外周血白细胞计数（×１０９／Ｌ）为３．４２±１．２０和５．５６±２．５０，显著高于对照组的２．０３±０．９０和３．７２±２．３０（Ｐ均＜０．０５）；照射后第１０，１５，２０，２５和３０天，治疗组小鼠外周血单核细胞计数（×１０９／Ｌ）为０．０８±０．０６，０．１６±０．１０，０．４８±０．３５，０．４７±０．２１和０．３３±０．１７，分别高于对照组的０．０２５±０．０１６，０．０５±０．０４，０．２３±０．１６，０．３３±０．１９和０．１７±０．１３（Ｐ均＜０．０５）；照射后第１５，２０和２５天，治疗组小鼠外周血中性粒细胞数（×１０９／Ｌ）为１．０３±０．６１，２．１８±１．１９和３．２８±１．０９，也高于对照组的０．６２±０．３７，１．４０±０．９９和２．２０±０．７４（Ｐ均＜０．０５）；照射后第９天骨髓ＣＦＵ－ＧＭ集落形成试验显示对照组的集落数（１９±１１／?

Objective:To demonstrate the hematopoietic enhancement activity of recombinant human macrophage colony stimulating factor (rhM CSF). Methods:Balb/c mice were sublethally irradiated with 60 Co γ rays and then the rhM CSF expressed in silkworm (10 3U per individual for 7 days) was administered intraperitoneally.Results:①The peripheral white blood cell counts of treatment group at day 15 and day 20 after irradiation were significantly higher than those of normal control group;②At days 10,15,20,25 and 30 after irradiation,the difference between the peripheral monocyte counts of the treatment and control group were statistically significant;③At days 15,20 and 25 after irradiation, the difference between the peripheral granulocyte counts of the two groups were also significant;④At day 9 after irradiation,the bone marrow CFU GM yield of the control group were significantly lower than those of the treatment group.Conclusion:rhM CSF expressed in silkworm could accelerate hematopoietic recovery in irradiated mice.