期刊文献+

短程大剂量粒细胞集落刺激因子动员外周血造血干细胞的研究 被引量:6

Studies on peripheral blood stem cells mobilization by short course and high dose G CSF
原文传递
导出
摘要 目的:研究短程大剂量粒细胞集落刺激因子(G-CSF)对外周血造血干细胞的动员作用。方法:采用短程大剂量G-CSF对11例患者进行外周血造血干细胞动员,G-CSF5μg/kg皮下注射,每日2次,共3天,动员当天及第4天(单采日),分别取骨髓及外周血进行常规检查,CFU-GM、BFU-E、CFU-mix集落培养及CD34、CD38细胞检测。结果:动员后患者骨髓增生明显活跃,外周血白细胞计数明显升高。骨髓、外周血CFU-GM、BFU-E及CFU-mix集落,CD34+CD38+细胞、CD34+CD38-细胞均较动员之前明显增多(P<0.05)。一次单采的单个核细胞(MNC)平均为1.56×108/kg。冷冻后MNC、CFU-GM、BFU-E及CD34+CD38+细胞的恢复率为80%(P>0.05);CFU-mix的恢复率为60%,但CD34+CD38-细胞冷冻后减少(P<0.05)。回输患者体内MNC的平均集落数分别为:CFU-GM6.70×104/kg;BFU-E1.60×104/kg;CFU-mix0.32×104/kg;CD34+CD38+细胞1.60×106/kg;CD34+CD38+细胞0.23×106? Objective:To explore the effect of short course,high dose granulocyte colony stimulating factor (G CSF) on peripheral blood stem cell(PBSC) mobolization.Methods:G CSF 5μg/kg was injected subcutaneously twice daily for 3 consecutive days.On the first day and the fourth day the peripheral blood and bone marrow were collected for CFU GM,BFU E,CFU mix assays and CD 34 /CD 38 detection.A single apheresis of over 8000ml blood processing was performed with CS 3000 blood cell separator.The mononuclear cells were prepared for hematopoietic colony assays and CD 34 /CD 38 detection before and after freezing.Results:After adminitration of G CSF, the white blood cell count increased significantly.The CFU GM,BFU E,CFU mix yields and CD 34 + CD 38 + cell and CD 34 + CD 38 - cells also increased significantly (P<0.05).The median of mononuclear cells was 1.56×10 8/kg.After one week freezing ,the recovery of mononuclear cells,CFU GM,BFU E yields and CD 34 + CD 38 + cells were about 80%(P>0.05) and CFU mix yields was 60%; The CD 34 + CD 38 - cells were decreased compared with that of pre freezing (P <0 05).The median of the reinfused mononuclear cells was 1.27×10 8/kg,CFU GM 6.7×10 4/kg,BFU E 1.6×10 4/kg,CFU mix 0.32×10 4/kg, CD 34 + CD 38 + cells 1.6×10 6/kg and CD 34 + CD 38 - cells 0.23×10 6 /kg.Conclusion:Short course and high dose G CSF can effectively mobilize the peripheral stem cells.The mononuclear cells,hematopoietic colonies and CD 34 /CD 38 cells were satisfied for the peripheral stem cells rescue.
出处 《中华血液学杂志》 CAS CSCD 北大核心 1997年第6期287-290,共4页 Chinese Journal of Hematology
  • 引文网络
  • 相关文献

同被引文献39

  • 1冯茹,周淑芸,袁进.G-CSF和DS动员干细胞作用与不同来源干细胞移植的比较[J].中国实验血液学杂志,1996,4(3):319-323. 被引量:1
  • 2范文茂,陆东风,张东成,赖永东.人重组粒细胞刺激因子治疗动物模型急性心肌梗死的病理学观察[J].血栓与止血学,2004,10(4):172-174. 被引量:4
  • 3张力,管忠震.国产 rhG-CSF 预防肿瘤化疗所致白细胞减少的Ⅱ期临床研究[J].癌症,1997,16(3):206-210. 被引量:12
  • 4Iwanage K, Takano H, Ohtsuka M, etal. Effects of G-CSF on cardiac remodeling after acute myocardial infarction in swine[J]. Biochem Biochys Res Commun, 2004, 325(4):1353-1359.
  • 5Kronenwett R, Martin S, Haas R. The role of cytokines and adhesion molecules for mobilization of peripheral blood stem cells[J]. Stem Cells, 2000, 18(5) : 320-330.
  • 6Levi-Schaffer F, Garbuzenko E, Rubin A,et al. Human eosinophils regulate human lung-and skin-derived fibroblast properties in vitro: a role for transforming growth factor beta (TGF-beta) [J]. Proc Natl Acad Sci U S A,1996,96(17):9660-9665.
  • 7Lichterfeld M, Martin S,Burkly L,et al. Mobilization of CD34 + haematopoietic stem cells is associated with a functional inactivation of the integrin very late antigen 4 [J].Br J Hae matol, 2000 , 110(1):71-81.
  • 8Levesque JP, Takamatsu Y, Nilsson SK, et al. Vascular cell adhesion molecule-1 (CD106)is cleaved by neutrophil proteases in the bone marrow following hematopoietic progenitor cell mobilization by granulocyte colony-stimulating factor [J].Blood,2001, 98(5) :1289-1297.
  • 9Goldman BI, Wurzel J. Evidence that human cardiac myocytes divide after myocardial infarction [J]. N Engl J Med, 2001,345(15): 1131-1131.
  • 10Dyson PG, Jackson KA,McClure BJ, et al. Increased levels of megakaryocyte progenitors in peripheral blood mobilised by chemotherapy and/or haemopoietic growth factor protocols[J].Bone Marrow Transplant, 1996,18(4) :705-712.

引证文献6

二级引证文献3

;
使用帮助 返回顶部