摘要
目的:研究短程大剂量粒细胞集落刺激因子(G-CSF)对外周血造血干细胞的动员作用。方法:采用短程大剂量G-CSF对11例患者进行外周血造血干细胞动员,G-CSF5μg/kg皮下注射,每日2次,共3天,动员当天及第4天(单采日),分别取骨髓及外周血进行常规检查,CFU-GM、BFU-E、CFU-mix集落培养及CD34、CD38细胞检测。结果:动员后患者骨髓增生明显活跃,外周血白细胞计数明显升高。骨髓、外周血CFU-GM、BFU-E及CFU-mix集落,CD34+CD38+细胞、CD34+CD38-细胞均较动员之前明显增多(P<0.05)。一次单采的单个核细胞(MNC)平均为1.56×108/kg。冷冻后MNC、CFU-GM、BFU-E及CD34+CD38+细胞的恢复率为80%(P>0.05);CFU-mix的恢复率为60%,但CD34+CD38-细胞冷冻后减少(P<0.05)。回输患者体内MNC的平均集落数分别为:CFU-GM6.70×104/kg;BFU-E1.60×104/kg;CFU-mix0.32×104/kg;CD34+CD38+细胞1.60×106/kg;CD34+CD38+细胞0.23×106?
Objective:To explore the effect of short course,high dose granulocyte colony stimulating factor (G CSF) on peripheral blood stem cell(PBSC) mobolization.Methods:G CSF 5μg/kg was injected subcutaneously twice daily for 3 consecutive days.On the first day and the fourth day the peripheral blood and bone marrow were collected for CFU GM,BFU E,CFU mix assays and CD 34 /CD 38 detection.A single apheresis of over 8000ml blood processing was performed with CS 3000 blood cell separator.The mononuclear cells were prepared for hematopoietic colony assays and CD 34 /CD 38 detection before and after freezing.Results:After adminitration of G CSF, the white blood cell count increased significantly.The CFU GM,BFU E,CFU mix yields and CD 34 + CD 38 + cell and CD 34 + CD 38 - cells also increased significantly (P<0.05).The median of mononuclear cells was 1.56×10 8/kg.After one week freezing ,the recovery of mononuclear cells,CFU GM,BFU E yields and CD 34 + CD 38 + cells were about 80%(P>0.05) and CFU mix yields was 60%; The CD 34 + CD 38 - cells were decreased compared with that of pre freezing (P <0 05).The median of the reinfused mononuclear cells was 1.27×10 8/kg,CFU GM 6.7×10 4/kg,BFU E 1.6×10 4/kg,CFU mix 0.32×10 4/kg, CD 34 + CD 38 + cells 1.6×10 6/kg and CD 34 + CD 38 - cells 0.23×10 6 /kg.Conclusion:Short course and high dose G CSF can effectively mobilize the peripheral stem cells.The mononuclear cells,hematopoietic colonies and CD 34 /CD 38 cells were satisfied for the peripheral stem cells rescue.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1997年第6期287-290,共4页
Chinese Journal of Hematology