集安红参HPLC数字化指纹图谱研究

Digitized fingerprints of Panax Ginseng rubra by HPLC

目的:建立红参HPLC数字化指纹图谱,为红参质量控制提供依据。方法:利用反相HPLC法分析红参75(V/V)乙醇提取物,采用CenturySILC18AQ柱(25cm×4.6mm,5μm),流动相为0.1mol/LNaH2PO4水溶液-乙腈,线性梯度洗脱,检测波长203nm,柱温(30±0.15)℃,进样量20μL。以色谱指纹图谱指数F,定性相似度SF和定量相似度P等数字化指标进行评价。结果:以腺苷峰为参照物峰,确定30个共有峰,充分揭示了红参HPLC指纹图谱的超信息特征。结论:所建立的指纹图谱的特征性、专属性强,具有很好的精密度和重复性,适于红参质量控制。

AIM： To establish a HPLC digitized fingerprints of Panax Ginseng rubra to control its quality. METHODS： The chromatographic fingerprints were determined by injecting 20 μL of the sample solution each time on a Century SIL C18 AQ column （20 cm ×4.6 mm, 5 μm） with the gradient elution solvent system composed of 0.1 mol/L NaH2PO4 aqueous solution-acetonitrile. The flow rate was 1 mL/min, the column temperature was maintained at （30 ± 0. 15 ） ℃ and the signals were acquired at 203 nm. The chromatographic fingerprints were evaluated by both the chromatographic fingerprint index （F） and the information index of chromatographic fingerprint （I） and so on. RESULTS： 30 co-possessing peaks were selected as the fingerprint peaks and the similarities between each of the ten batches and the referential chromatographic fingerprints of Panax Ginseng rubra were calculated while taking adenosine peak as the reference peak. CONCLUSION： This method with good characteristics and specificity can be used in the quality control of Panax Ginseng rubra.