数字化色谱指纹谱法控制半夏的质量

Quality control of Rhizoma Pinelliae by chromatograph-digitized fingerprint spectrum

目的:建立半夏的数字化色谱指纹谱,以对其进行质量控制。方法:采用HPLC梯度洗脱技术进行分离分析,色谱柱:AgilentZorbaxODSC18柱;流动相:乙腈-水作梯度洗脱;检测波长:210nm;流速:0.5mL/min;柱温:25℃。结果:在选定的色谱条件下,建立了半夏的数字化色谱指纹谱,制定了控制半夏质量的指纹谱技术参数。结论:利用数字化色谱指纹谱技术控制半夏的质量是可行的。

AIM： To establish HPLC-digitized fingerprint spectrum （HPLC-DFPS） of Rhizoma Pinelliae so that it will be applied in quality control of Rhizoma Pinelliae. METHODS ： A gradient separated method was applied. Column： Agilent Zorbax ODS C18; Mobile phase： acetonitrile-water; Detection-wavelength： 210 nm; Flow rate： 0.5 mL/min; Column temperature： 25 ℃. RESULTS： Using above optimum chromatograph condition, the HPLC- DFPS and technic parameters of Rhizoma Pinelliae were established. CONCLUSION： Comparison of a group of characteristic peaks suggests that the HPLC-DFPS technology is feasible in quality control of Rhizoma Pinelliae.