甘草中黄酮的纯化方法和含量测定

Purification and assay of flavonoids in glycyrrhiza

目的:研究聚酰胺富集甘草中黄酮的工艺参数,同时建立其含量测定方法。方法:以二氢黄酮类化合物在碱性条件下转化为查尔酮类的量作为指标,以柚皮苷为对照品,经聚酰胺吸附柱富集后,采用碱性比色法显色、测定。结果:纯化工艺条件为甘草聚酰胺比2∶1(g/g),树脂径高比1∶7,用5倍量柱体积70乙醇洗脱,甘草黄酮洗脱率在90左右;含量测定平均加样回收率为98.81(RSD=2.00)。总固物中黄酮含量可达45。结论:本法富集、纯化甘草总黄酮可行;甘草黄酮含量测定方法稳定,可靠。

AIM：To investigate the process parameters of adsorbing and separating the total flavones of Radix et Rhizoma glycyrrhiae and its assay. METHODS： Chalcone that flavanone in Licorice root was converted into in alkaline condition was adopted as index and niaringoside as control, through polyamide treatment, alkaline colorimetty was used to determine chalcone content. RESULTS ： Under the condtion, namely, ratio of quantity of Licorice root to polyamide was 2：1, ratio of column diameter to column heighth was 1：7,70% ethanol used to eluate, flavone extract was up to 45%with 90% eluting yield. The recoveny of assaying was 98.17% （RSD = 2.00% ）. CONCLUSION： The purification and assay of Licorice flavonoids are reliable and efficacious.